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Rabbit Recombinant Monoclonal YKL-40/CHI3L1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB305035), expandable thumbnail
  • Immunocytochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB305035), expandable thumbnail
  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB305035), expandable thumbnail
  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB305035), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (AB305035), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Alternative names

Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1

Recommended products

Rabbit Recombinant Monoclonal YKL-40/CHI3L1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27396-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.

Biological function summary

YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.

Pathways

YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.

Associated diseases and disorders

YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg

    Lane 2: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa

    Observed band size: 42 kDa

    Exposure time: 180s

  • Immunocytochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Immunocytochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling YKL-40/CHI3L1 with ab305035 at 1/50 (10.92 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell line.Negative control: A-204.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative controls: A-204, Jurkat (PMID: 16472595)
    Exposure time: 48 seconds

    All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

    Lane 2: A-204 (human muscle rhabdomyosarcoma cell), whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa

    Observed band size: 42 kDa

    Exposure time: 48s

  • Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human CHIT1 or CHI3L2.
    Exposure time: 8 seconds

    All lanes: Western blot - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035) at 1/1000 dilution

    Lane 1: Human CHI3L1 recombinant protein (No tag, aa22-383) at 10 ng

    Lane 2: 293T transfected with human CHITI (aa 22-466) expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T transfected with human CHI3L2 (aa 27-390) expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa

    Observed band size: 42 kDa

    Exposure time: 8s

  • Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A-204 (human muscle rhabdomyosarcoma, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling YKL-40/CHI3L1 with ab305035 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: A-204.

  • Immunohistochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Immunohistochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling YKL-40/CHI3L1 with ab305035 at 1/500 (1.092 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human glioma. The section was incubated with ab305035 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035), expandable thumbnail

    Immunohistochemistry - Anti-YKL-40/CHI3L1 antibody [EPR27396-56] (ab305035)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling YKL-40/CHI3L1 with ab305035 at 1/500 (1.092 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab305035 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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