AB319165

Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
KO Validated
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Rabbit Recombinant Multiclonal YKL-40/CHI3L1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1, Brp39, Chil1, Chi3l1, Chitinase-3-like protein 1, BRP39 protein, Breast regression protein 39, Cartilage glycoprotein 39, CGP-39, GP-39

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) (Left) / CHI3L1 KO THP-1(FR090372) (Right) cells labelling YKL-40/CHI3L1 with ab319164 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of rat lung. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of mouse colon cancer. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of mouse lung. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling YKL-40/CHI3L1 with ab319164 at 1/500 (1.01 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat spleen. The section was incubated with ab319164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

MSD279 antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Negative control : 293T, Jurkat (PMID : 19767768, PMID : 16472595).

The molecular weight observed is consistent with what has been described in the literature (PMID : 19414556).

The identity of the lower MW band at approximately 25 kDa (in lane 3 and 5) is unknown.

In Western blot, ab319164 was shown to bind specifically to CHI3L1. Target of interest was observed at 43 kDa in wild-type THP-1 cell lysates (lane 1) with no signal observed at this size in CHI3L1 knockout cell line (lane 2, knockout cell line ab280038).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : lane 1-2 : 25 seconds, lane 3-5 : 125 seconds

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] (<a href='/en-us/products/primary-antibodies/ykl-40-chi3l1-antibody-rm1204-ab319164'>ab319164</a>) at 1/1000 dilution

Lane 1:

Wide-type THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

CHI3L1 knockout THP-1 whole cell lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa,36 kDa

false

Supplier Data

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

This data was developed using ab319164, the same antibody clone in a different buffer formulation.

Low expression tissue : liver (PMID : 31250532).

The molecular weight observed is consistent with what has been described in the literature (PMID : 19414556).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] (<a href='/en-us/products/primary-antibodies/ykl-40-chi3l1-antibody-rm1204-ab319164'>ab319164</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa,36 kDa

false

Exposure time: 37s

Unconjugated

Anti-YKL-40/CHI3L1 antibody [RM1204]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1204

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab319165 is the carrier-free version of ab319164.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.

Biological function summary

YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.

Pathways

YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.

YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

See full target information CHI3L1

Additional targets

Chi3l1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Flow Cytometry (Intracellular) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Western blot - Anti-YKL-40/CHI3L1 antibody [RM1204] - BSA and Azide free (AB319165)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise