AB241382

Anti-YKT6 antibody

Name: Anti-YKT6 antibody (ab241382) | Abcam
Brand: Abcam Limited
SKU: AB241382
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal YKT6 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus.

View Alternative Names

Synaptobrevin homolog YKT6, YKT6

2 Images

Supplier Data

Immunoprecipitation - Anti-YKT6 antibody (AB241382)

YKT6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241382 used for IP at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241382 at 1 μg/ml.

Lane 1 : ab241382 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-YKT6 antibody (ab241382)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-YKT6 antibody (AB241382)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-YKT6 antibody (ab241382) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 22 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241382 was affinity purified using an epitope specific to YKT6 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YKT6 also known as Synaptobrevin-like 2 is a SNARE protein involved in vesicular transport. YKT6 has a molecular mass of about 23 kDa and is present across various tissues notably in the brain and pancreas. This protein does not have a transmembrane domain which allows cycling between membrane-bound and cytosolic forms contributing to its role in membrane fusion processes.

Biological function summary

YKT6 aids in the regulation of membrane fusion events important for the secretory pathway. It operates as part of the SNARE complex which includes other proteins like VAMPs syntaxin and SNAP-25. These components form a tight complex that brings vesicle and target membranes into close proximity driving the fusion necessary for intracellular transport and secretion.

Pathways

YKT6 connects to exocytosis and endocytosis pathways which are important for neurotransmitter release and cellular uptake processes. It interacts with proteins such as SNAP-25 and syntaxins within these pathways. As a SNARE protein YKT6's role in vesicle trafficking is vital for maintaining cellular homeostasis and regulating signal transmission across synapses.

Abnormalities in YKT6 expression or function impact neurodegenerative diseases like Alzheimer's disease. Altered interactions between YKT6 and other proteins such as syntaxin can disrupt normal synaptic function and contribute to pathogenesis. Additionally irregular YKT6 activity may play a role in certain cancers where vesicular transport and secretion are typically disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.

See full target information Synaptobrevin homolog YKT6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe

Nature communications 13:4918 PubMed35995799

2022

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xue Xie,Nima N Naseri,Jasmine Fels,Parinati Kharel,Yoonmi Na,Diane Lane,Jacqueline Burré,Manu Sharma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-YKT6 antibody

Immunoprecipitation - Anti-YKT6 antibody (AB241382)

Western blot - Anti-YKT6 antibody (AB241382)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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