Rabbit Polyclonal YKT6 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15498
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.
Alternative names
Synaptobrevin homolog YKT6, YKT6
Recommended products
Rabbit Polyclonal YKT6 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Synaptobrevin homolog YKT6 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15498
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab241382 was affinity purified using an epitope specific to YKT6 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YKT6 also known as Synaptobrevin-like 2 is a SNARE protein involved in vesicular transport. YKT6 has a molecular mass of about 23 kDa and is present across various tissues notably in the brain and pancreas. This protein does not have a transmembrane domain which allows cycling between membrane-bound and cytosolic forms contributing to its role in membrane fusion processes.
- Biological function summary
YKT6 aids in the regulation of membrane fusion events important for the secretory pathway. It operates as part of the SNARE complex which includes other proteins like VAMPs syntaxin and SNAP-25. These components form a tight complex that brings vesicle and target membranes into close proximity driving the fusion necessary for intracellular transport and secretion.
- Pathways
YKT6 connects to exocytosis and endocytosis pathways which are important for neurotransmitter release and cellular uptake processes. It interacts with proteins such as SNAP-25 and syntaxins within these pathways. As a SNARE protein YKT6's role in vesicle trafficking is vital for maintaining cellular homeostasis and regulating signal transmission across synapses.
- Associated diseases and disorders
Abnormalities in YKT6 expression or function impact neurodegenerative diseases like Alzheimer's disease. Altered interactions between YKT6 and other proteins such as syntaxin can disrupt normal synaptic function and contribute to pathogenesis. Additionally irregular YKT6 activity may play a role in certain cancers where vesicular transport and secretion are typically disrupted.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-YKT6 antibody (ab241382)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-YKT6 antibody (ab241382) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 3min
Immunoprecipitation - Anti-YKT6 antibody (ab241382)
YKT6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241382 used for IP at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241382 at 1 μg/ml.Lane 1: ab241382 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-YKT6 antibody (ab241382)
Predicted band size: 22 kDa
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