AB112055

Anti-YL1 antibody

Name: Anti-YL1 antibody (ab112055) | Abcam
Brand: Abcam Limited
SKU: AB112055
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal YL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human VPS72 aa 50-100.

View Alternative Names

TCFL1, YL1, VPS72, Vacuolar protein sorting-associated protein 72 homolog, Protein YL-1, Transcription factor-like 1

2 Images

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Immunoprecipitation - Anti-YL1 antibody (AB112055)

Immunoprecipitation of YL1. Samples : 1mg of whole cell lysate from HeLa cells(20% of IP loaded). Antibodies : Affinity purified rabbit anti-YL1 antibody (ab112055) was used at 1 μg/ml. Lane 1- YL1 was also immunoprecipitated by rabbit anti-YL1 antibody (ab112055) at 6 μg/mg lysate. Lane 2- YL1 was immunoprecipitated by an rabbit anti-YL1 antibody that recognizes a downstream epitope. 3- Rabbit IgG control.Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-YL1 antibody (ab112055)

Predicted band size: 41 kDa

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Western blot - Anti-YL1 antibody (AB112055)

All lanes:

Western blot - Anti-YL1 antibody (ab112055) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human VPS72 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q15906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Baboon": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein YL1 also known as Tyrosine-tubulin ligase-like family member 5 (TUBE1) plays an important role in regulating microtubule dynamics. YL1 has a molecular mass of approximately 14 kDa. It predominantly expresses in cells with high microtubule activity such as neural tissues and dividing cells. The protein actively binds to tubulin which is an essential component of the microtubule network within the cell. This binding modifies tubulin dynamics impacting cell shape and intracellular transport.

Biological function summary

YL1 influences the stability and organization of microtubules within the cellular cytoskeleton. It is part of a functional complex that involves tubulin which facilitates proper microtubule attachment and detachment during cell division. By modulating microtubule filament properties YL1 ensures accurate chromosome segregation contributing to the faithful completion of mitosis and meiosis.

Pathways

YL1 takes part in important cellular processes related to the regulation of cytoskeleton dynamics and cell cycle progression. It actively participates in the mitotic spindle assembly pathway where it cooperates with proteins such as TPX2 and Aurora A kinase. Additionally YL1 associates with the Rho GTPase signaling pathway which affects cytoskeletal reorganization and cell morphology linking to proteins like RhoA and Rac1 vital for actin and microtubule coordination.

YL1 has shown implications in conditions such as cancer and neurodegenerative diseases. Dysregulation of YL1 and associated pathways may contribute to uncontrolled cell proliferation and tumor development with connections to proteins like p53 and BRCA1. In neurodegenerative disorders abnormal YL1 function might affect neuronal microtubule integrity associating it with proteins such as tau which is involved in tauopathies like Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deposition-and-exchange histone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. As component of the SRCAP complex, mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling.

See full target information VPS72

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 44:72-85 PubMed38482865

2024

ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Dijkwel,Gene Hart-Smith,Sebastian Kurscheid,David J Tremethick

Bioengineered 13:9197-9210 PubMed35383533

2022

Vacuolar protein sorting-associated protein 72 homolog (VPS72) binding to lysine acetyltransferase 5 (KAT5) promotes the proliferation, invasion and migration of hepatocellular carcinoma through regulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tiejun Chen,Yinuo Tu,Dongnuo Lv,Kunpeng Lin,Hui Tang,Wenjie Huang

Nature communications 10:1071 PubMed30842416

2019

Znhit1 controls intestinal stem cell maintenance by regulating H2A.Z incorporation.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhao,Ying Chen,Ning Jiang,Li Yang,Shenfei Sun,Yan Zhang,Zengqi Wen,Lorraine Ray,Han Liu,Guoli Hou,Xinhua Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-YL1 antibody

Immunoprecipitation - Anti-YL1 antibody (AB112055)

Western blot - Anti-YL1 antibody (AB112055)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise