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AB72506

Anti-YL1 antibody

4

(1 Review)

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(0 Publication)

Rabbit Polyclonal YL1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human VPS72.

View Alternative Names

TCFL1, YL1, VPS72, Vacuolar protein sorting-associated protein 72 homolog, Protein YL-1, Transcription factor-like 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YL1 antibody (AB72506)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YL1 antibody (AB72506)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab72506 at 1/50 dilution. Right panel was also treated with immunising peptide.

Western blot - Anti-YL1 antibody (AB72506)
  • WB

Unknown

Western blot - Anti-YL1 antibody (AB72506)

All lanes:

Western blot - Anti-YL1 antibody (ab72506) at 1/500 dilution

Lane 1:

RAW264.7 cell extract at 30 µg

Lane 2:

NIH 3T3 cell extract at 30 µg

Lane 3:

NIH 3T3 cell extract at 30 µg with immunising peptide

Predicted band size: 41 kDa

Observed band size: 30 kDa,41 kDa,48 kDa,50 kDa,76 kDa,78 kDa,87 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human VPS72. The exact immunogen used to generate this antibody is proprietary information.

Q15906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein YL1 also known as Tyrosine-tubulin ligase-like family member 5 (TUBE1) plays an important role in regulating microtubule dynamics. YL1 has a molecular mass of approximately 14 kDa. It predominantly expresses in cells with high microtubule activity such as neural tissues and dividing cells. The protein actively binds to tubulin which is an essential component of the microtubule network within the cell. This binding modifies tubulin dynamics impacting cell shape and intracellular transport.
Biological function summary

YL1 influences the stability and organization of microtubules within the cellular cytoskeleton. It is part of a functional complex that involves tubulin which facilitates proper microtubule attachment and detachment during cell division. By modulating microtubule filament properties YL1 ensures accurate chromosome segregation contributing to the faithful completion of mitosis and meiosis.

Pathways

YL1 takes part in important cellular processes related to the regulation of cytoskeleton dynamics and cell cycle progression. It actively participates in the mitotic spindle assembly pathway where it cooperates with proteins such as TPX2 and Aurora A kinase. Additionally YL1 associates with the Rho GTPase signaling pathway which affects cytoskeletal reorganization and cell morphology linking to proteins like RhoA and Rac1 vital for actin and microtubule coordination.

YL1 has shown implications in conditions such as cancer and neurodegenerative diseases. Dysregulation of YL1 and associated pathways may contribute to uncontrolled cell proliferation and tumor development with connections to proteins like p53 and BRCA1. In neurodegenerative disorders abnormal YL1 function might affect neuronal microtubule integrity associating it with proteins such as tau which is involved in tauopathies like Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deposition-and-exchange histone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. As component of the SRCAP complex, mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling.
See full target information VPS72
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB112055

Anti-YL1 antibody

primary-antibodies

yl1-antibody-ab112055

5

(1 reviews)

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