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AB225950

Anti-YopM antibody

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(1 Publication)

Rabbit Polyclonal YOPM antibody. Suitable for WB and reacts with Recombinant full length protein - Yersinia pestis samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Yersinia pestis yopM.

View Alternative Names

yop48, YPCD1.26c, y5054, y0059, YP_pCD60, yopM, Outer membrane protein YopM

1 Images
Western blot - Anti-YopM antibody (AB225950)
  • WB

Supplier Data

Western blot - Anti-YopM antibody (AB225950)

All lanes:

Western blot - Anti-YopM antibody (ab225950) at 1/500 dilution

Lane 1:

Recombinant protein 80 ng

Lane 2:

Recombinant protein 40 ng

Lane 3:

Recombinant protein 20 ng

Lane 4:

Recombinant protein 10 ng

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Yersinia pestis yopM.

P17778

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Primary Antibodies

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Primary Antibodies

AB225998

Anti-Osmotin like protein OSM34 antibody

Western blot - Anti-Osmotin like protein OSM34 antibody (AB225998)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Yersinia pestis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Yersinia pestis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YopM also known as Yersinia outer protein M functions as an important virulence factor used by the Yersinia bacteria. It is a protein with a molecular mass of approximately 40 kDa. Through Type III secretion system Yersinia bacteria inject YopM into host cells. It localizes predominantly within the cytosol and the nucleus of the host cells after injection. YopM operates by altering host cellular pathways to promote bacterial survival and replication.
Biological function summary

YopM manipulates host immune responses by interfering with signal transduction processes. It forms a complex with several host proteins including RSK1 (ribosomal S6 kinase 1) and PRK2 (protein kinase C-related kinase 2). These interactions help modulate inflammatory pathways within the host by inhibiting protein kinases that are important for immune response. The host-pathogen interaction mediated through YopM is vital for Yersinia's ability to subvert the host's immune system effectively.

Pathways

YopM plays a significant role in the MAPK (mitogen-activated protein kinase) signaling pathway. This involvement disrupts normal host cell signaling events that would typically result in the activation of defense responses. Interaction with the RSK and PRK protein kinases influences cell survival and proliferation pathways. These interactions inhibit essential signaling cascades therefore facilitating the bacteria's effective evasion of the host's innate immune response.

YopM's role is associated with conditions that result from infections with Yersinia species such as Yersinia pestis which causes plague. YopM's manipulation of immune pathways links it to the immune-evasive properties seen in these bacterial infections. Researchers have studied YopM's connection to the interleukin signaling alterations as it affects interleukin production which is an important factor in inflammatory diseases when improperly regulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues.
See full target information yopM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:1257-1272 PubMed38454149

2024

Structure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport.

Applications

Unspecified application

Species

Unspecified reactive species

Aakash G Mukhopadhyay,Katerina Toropova,Lydia Daly,Jennifer N Wells,Laura Vuolo,Miroslav Mladenov,Marian Seda,Dagan Jenkins,David J Stephens,Anthony J Roberts
View all publications
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Product promise

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