Anti-YTHDC1 antibody

Target data

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed : 25242552, PubMed : 26318451, PubMed : 26876937, PubMed : 28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed : 25242552, PubMed : 26318451). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed : 26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed : 26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping : this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed : 26876937). May also regulate alternative splice site selection (PubMed : 20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3 : interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (PubMed : 27602518). Involved in random X inactivation mediated by Xist RNA : recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed : 27602518). Also recognizes and binds m6A-containing single-stranded DNA (PubMed : 32663306). Involved in germline development : required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity).