Anti-YTHDC1 antibody [EPR21821-213] (ab259990)

Rabbit Recombinant Monoclonal YTHDC1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 17 publications.

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Images

Western blot - Anti-YTHDC1 antibody [EPR21821-213] (AB259990), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	Flow Cyt (Intra)	IHC-P
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Tested	Not recommended	Tested	Tested	Tested
Rat	Expected	Not recommended	Tested	Expected	Tested
Cat	Predicted	Not recommended	Predicted	Predicted	Predicted
Common marmoset	Predicted	Not recommended	Predicted	Predicted	Predicted
Cynomolgus monkey	Predicted	Not recommended	Predicted	Predicted	Predicted
Dog	Predicted	Not recommended	Predicted	Predicted	Predicted
Pig	Predicted	Not recommended	Predicted	Predicted	Predicted
Rhesus monkey	Predicted	Not recommended	Predicted	Predicted	Predicted
Xenopus tropicalis	Predicted	Not recommended	Predicted	Predicted	Predicted
Zebrafish	Predicted	Not recommended	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -
Species Cat	Dilution info -	Notes -
Species Dog	Dilution info -	Notes -
Species Pig	Dilution info -	Notes -
Species Zebrafish	Dilution info -	Notes -
Species Cynomolgus monkey	Dilution info -	Notes -
Species Rhesus monkey	Dilution info -	Notes -
Species Common marmoset	Dilution info -	Notes -
Species Xenopus tropicalis	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis	Dilution info -	Notes -

Target data

See full target information YTHDC1

Function

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:25242552, PubMed:26318451, PubMed:26876937, PubMed:28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:25242552, PubMed:26318451). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518). Also recognizes and binds m6A-containing single-stranded DNA (PubMed:32663306). Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity).

Alternative names

KIAA1966, YT521, YTHDC1, YTH domain-containing protein 1, Splicing factor YT521, YT521-B

Recommended products

Rabbit Recombinant Monoclonal YTHDC1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 17 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21821-213
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

YTHDC1 also known as YT521-B is a nuclear protein involved in mRNA processing. It belongs to the YTH domain family recognized for binding m6A-modified RNA. The protein has a mass of approximately 82 kDa. Expression of YTHDC1 occurs mainly in the nucleus but may also be found in other cellular compartments. Its widespread presence includes tissues such as the brain and liver suggesting its diverse functional roles.

Biological function summary

YTHDC1 operates in the regulation of mRNA splicing and transportation. It associates with spliceosome components to influence alternative splicing decisions. YTHDC1 part of the m6A reader complex recognizes the m6A methylation mark on RNA aiding in the control of mRNA fate. Additionally its interactions with certain proteins affect RNA stability and translation essential for proper gene expression regulation.

Pathways

YTHDC1 plays a significant role in RNA metabolism and the cellular response to stress. Involvement in the mRNA splicing pathway highlights its contribution to gene expression regulation. YTHDC1 interacts with proteins like SRSF3 and SRFS10 which modulate RNA splicing and stability. Another pathway includes its role in the cellular stress response where YTHDC1 helps maintain RNA integrity linking it to adaptive responses in cells.

Associated diseases and disorders

YTHDC1 captures interest regarding cancer and neurodegenerative diseases. Abnormal expression levels or mutations in YTHDC1 have associations with various cancer types hinting at its role in tumorigenesis. Alterations in YTHDC1 expression impact splicing patterns and gene expression potentially influencing cancer progression. Additionally YTHDC1's connection to neurodegeneration involves proteins such as FUS and TDP-43 which are associated with disorders like amyotrophic lateral sclerosis (ALS) suggesting that YTHDC1 may play a role in RNA metabolism dysregulation observed in these diseases.

Product promise

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9 product images

Western blot - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lanes 1-4: Merged signal (red and green). Green - ab259990 observed at 100 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab259990 Anti-YTHDC1 antibody [EPR21821-213] was shown to specifically react with YTHDC1 in wild-type ES-D3 cells. Loss of signal was observed when a knockout cell line was used. Wild-type and YTHDC1 knockout samples were subjected to SDS-PAGE. ab259990 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

The wild-type and YTHDC1 knockout ES-D3 lysates were kindly provided by an anonymous collaborator.
All lanes: Western blot - Anti-YTHDC1 antibody [EPR21821-213] (ab259990) at 1/1000 dilution
Lane 1: Wild-type ES-D3 (mouse embryonic pluripotent stem Cell) whole cell lysate at 20 µg
Lane 2: YTHDC1 knockout ES-D3 cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa
Western blot - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 15 seconds
All lanes: Western blot - Anti-YTHDC1 antibody [EPR21821-213] (ab259990) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling YTHDC1 with ab259990 at 1/500 (1.152 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human testis. The section was incubated with Anti-YTHDC1 antibody [EPR21821] ab220159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling YTHDC1 with ab259990 at 1/500 (1.152 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse testis. The section was incubated with Anti-YTHDC1 antibody [EPR21821] ab220159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling YTHDC1 with ab259990 at 1/500 (1.152 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat testis. The section was incubated with Anti-YTHDC1 antibody [EPR21821] ab220159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling YTHDC1 with ab259990 at 1/50 (11.52 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear staining in HeLa cells (PMID: 18669619) is observed.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling YTHDC1 with ab259990 at 1/50 (11.52 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear staining in NIH/3T3 cells (PMID: 18669619) is observed.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling YTHDC1 with ab259990 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-YTHDC1 antibody [EPR21821-213] (ab259990)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling YTHDC1 with ab259990 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.