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Rabbit Recombinant Monoclonal YTHDC1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-YTHDC1 antibody [EPR21821-213] - BSA and Azide free (AB282289), expandable thumbnail
  • Western blot - Anti-YTHDC1 antibody [EPR21821-213] - BSA and Azide free (AB282289), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] - BSA and Azide free (AB282289), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] - BSA and Azide free (AB282289), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody [EPR21821-213] - BSA and Azide free (AB282289), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Tested
Tested
Rat
Expected
Not recommended
Tested
Expected
Tested
Cat
Predicted
Not recommended
Predicted
Predicted
Predicted
Common marmoset
Predicted
Not recommended
Predicted
Predicted
Predicted
Cynomolgus monkey
Predicted
Not recommended
Predicted
Predicted
Predicted
Dog
Predicted
Not recommended
Predicted
Predicted
Predicted
Pig
Predicted
Not recommended
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Not recommended
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Not recommended
Predicted
Predicted
Predicted
Zebrafish
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Cat
Dilution info
-
Notes

-

Species
Dog
Dilution info
-
Notes

-

Species
Pig
Dilution info
-
Notes

-

Species
Zebrafish
Dilution info
-
Notes

-

Species
Cynomolgus monkey
Dilution info
-
Notes

-

Species
Rhesus monkey
Dilution info
-
Notes

-

Species
Common marmoset
Dilution info
-
Notes

-

Species
Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Cat, Dog, Pig, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Xenopus tropicalis
Dilution info
-
Notes

-

Target data

Function

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:25242552, PubMed:26318451, PubMed:26876937, PubMed:28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:25242552, PubMed:26318451). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity). Also recognizes and binds m6A on other RNA molecules (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518). Also recognizes and binds m6A-containing single-stranded DNA (PubMed:32663306). Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing (By similarity).

Alternative names

KIAA1966, YT521, YTHDC1, YTH domain-containing protein 1, Splicing factor YT521, YT521-B

Recommended products

Rabbit Recombinant Monoclonal YTHDC1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21821-213
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab282289 is the carrier-free version of Anti-YTHDC1 antibody [EPR21821-213] ab259990.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YTHDC1 also known as YT521-B is a nuclear protein involved in mRNA processing. It belongs to the YTH domain family recognized for binding m6A-modified RNA. The protein has a mass of approximately 82 kDa. Expression of YTHDC1 occurs mainly in the nucleus but may also be found in other cellular compartments. Its widespread presence includes tissues such as the brain and liver suggesting its diverse functional roles.

Biological function summary

YTHDC1 operates in the regulation of mRNA splicing and transportation. It associates with spliceosome components to influence alternative splicing decisions. YTHDC1 part of the m6A reader complex recognizes the m6A methylation mark on RNA aiding in the control of mRNA fate. Additionally its interactions with certain proteins affect RNA stability and translation essential for proper gene expression regulation.

Pathways

YTHDC1 plays a significant role in RNA metabolism and the cellular response to stress. Involvement in the mRNA splicing pathway highlights its contribution to gene expression regulation. YTHDC1 interacts with proteins like SRSF3 and SRFS10 which modulate RNA splicing and stability. Another pathway includes its role in the cellular stress response where YTHDC1 helps maintain RNA integrity linking it to adaptive responses in cells.

Associated diseases and disorders

YTHDC1 captures interest regarding cancer and neurodegenerative diseases. Abnormal expression levels or mutations in YTHDC1 have associations with various cancer types hinting at its role in tumorigenesis. Alterations in YTHDC1 expression impact splicing patterns and gene expression potentially influencing cancer progression. Additionally YTHDC1's connection to neurodegeneration involves proteins such as FUS and TDP-43 which are associated with disorders like amyotrophic lateral sclerosis (ALS) suggesting that YTHDC1 may play a role in RNA metabolism dysregulation observed in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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