Anti-YTHDC2 antibody [EPR21820-49]
- RabMAb
- Recombinant
- KO Validated
- What is this?
5
(4 Reviews)
|
(29 Publications)
Rabbit Recombinant Monoclonal YTHDC2 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 29 publications.
View Alternative Names
3'-5' RNA helicase YTHDC2, YTH domain-containing protein 2, hYTHDC2, YTHDC2
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling YTHDC2 with ab220160 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human testis (PMID : 28380054). Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IP
Unknown
Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
YTHDC2 was immunoprecipitated from 0.35 mg of HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab220160 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220160 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1 : HeLa whole cell lysate 10 μ (Input).
Lane 2 : ab220160IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220160 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.
All lanes:
Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)
Predicted band size: 160 kDa
Observed band size: 160 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse testis (PMID : 28380054). Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on rat testis. Nuclear counterstain : DAPI.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on mouse testis. Nuclear counterstain : DAPI.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Negative control : (PMID : 29087293)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on mouse heart. Nuclear counterstain : DAPI.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Negative control : (PMID : 29087293)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on rat heart. Nuclear counterstain : DAPI.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat testis (PMID : 28380054). Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- WB
Supplier Data
Western blot - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure times.
Lane 1 : 3 minutes; Lanes 2-3 : 37 seconds; Lane 4 : 3 minutes; Lane 5 : 37 seconds.
All lanes:
Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160) at 1/1000 dilution
Lane 1:
HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 4:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5:
PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
false
- WB
Supplier Data
Western blot - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)
Blocking and dilution bufer : 5% NFDM/TBST.
The wild-type and YTHDC2 knockout cell lysates were kindly provided by an anonymous collaborator.
ab220160 was shown to specifically react with YTHDC2 in wild-type mESC cells as signal was lost in YTHDC2 knockout cells. Wild-type and YTHDC2 knockout samples were subjected to SDS-PAGE. ab220160 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160) at 1/1000 dilution
Lane 1:
Wild-type mESC (mouse embryo stem cell) whole cell lysate at 40 µg
Lane 2:
YTHDC2 knockout mESC whole cell lysate at 40 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
false
Exposure time: 3min
Related conjugates and formulations (1)
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Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
YTHDC2 contributes to the regulation of mRNA stability and turnover within the cell. It may operate as part of a complex involved in post-transcriptional control. Through these actions YTHDC2 influences processes such as meiosis and the differentiation of germ cells supported by its association with m6A reader proteins. This association plays a role in the recognition of specific m6A marks critical for RNA dynamics.
Pathways
YTHDC2 integrates into the m6A-dependent RNA decay and translation machinery. One important pathway involving this protein is the m6A-mediated gene expression regulation pathway. Within this context YTHDC2 interacts closely with other m6A machinery components such as METTL3 and bridges mRNA modification with subsequent cellular outcomes. Another related pathway is spermatogenesis where the precise control by YTHDC2 is necessary for successful meiosis via interaction with various meiotic proteins.
Product protocols
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Target data
Publications (29)
Recent publications for all applications. Explore the full list and refine your search
Oncology letters 30:498 PubMed40917727
2025
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Cancer cell international 25:290 PubMed40722171
2025
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Clinical and translational medicine 15:e70388 PubMed40629911
2025
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Communications biology 8:457 PubMed40102597
2025
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Journal of hepatocellular carcinoma 11:2393-2411 PubMed39649245
2024
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Frontiers in immunology 15:1440398 PubMed39534605
2024
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Theranostics 14:5551-5570 PubMed39310099
2024
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Military Medical Research 11:22 PubMed38622688
2024
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Cellular & molecular biology letters 29:36 PubMed38486141
2024
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Epigenetics 19:2326868 PubMed38465865
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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