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Rabbit Recombinant Monoclonal YTHDC2 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.


Images

Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (AB220160), expandable thumbnail
  • Western blot - Anti-YTHDC2 antibody [EPR21820-49] (AB220160), expandable thumbnail
  • Western blot - Anti-YTHDC2 antibody [EPR21820-49] (AB220160), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160), expandable thumbnail

Publications

  • JCI insight 8:2023
    The m6A methyltransferase METTL16 negatively regulates MCP1 expression in mesenchymal stem cells during monocyte recruitment.
    Applications:
    WB
    Reactive species:
    Human
    Zhaoqiang Zhang,Zhongyu Xie,Jiajie Lin,Zehang Sun,Zhikun Li,Wenhui Yu,Yipeng Zeng,Guiwen Ye,Jinteng Li,Feng Ye,Zepeng Su,Yunshu Che,Peitao Xu,Chenying Zeng,Peng Wang,Yanfeng Wu,Huiyong Shen
    PubMed 36795489
  • Cell death & disease 14:1482023
    METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer.
    Applications:
    WB
    Reactive species:
    Human
    Kangyue Sun,Lu Chen,Yiwen Li,Bing Huang,Qun Yan,Changjie Wu,Qiuhua Lai,Yuxin Fang,Jianqun Cai,Yongfeng Liu,Junsheng Chen,Xinke Wang,Yuxuan Zhu,Shuyu Dong,Jieyu Tan,Aimin Li,Side Liu,Yue Zhang
    PubMed 36810285

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Not recommended
Expected
Tested
Tested
Mouse
Expected
Tested
Not recommended
Tested
Expected
Tested
Rat
Expected
Tested
Not recommended
Tested
Expected
Tested
Cow
Predicted
Predicted
Not recommended
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Not recommended
Predicted
Predicted
Predicted
Primates
Predicted
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Cow, Pig, Primates

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Cow, Pig, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/600

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow, Pig, Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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    1 products for Alternative Version

      Target data

      Function

      3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells (PubMed:26318451, PubMed:29033321, PubMed:29970596). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability (PubMed:26318451, PubMed:29033321). Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (By similarity). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease (PubMed:29033321). Required for both spermatogenesis and oogenesis (By similarity).

      Alternative names

      3'-5' RNA helicase YTHDC2, YTH domain-containing protein 2, hYTHDC2, YTHDC2

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      Rabbit Recombinant Monoclonal YTHDC2 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

      Alternative names

      3'-5' RNA helicase YTHDC2, YTH domain-containing protein 2, hYTHDC2, YTHDC2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR21820-49

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      YTHDC2 also known as YT521-B homology domain containing 2 functions as an RNA helicase with a molecular mass of approximately 160 kDa. This protein modulates RNA metabolism by promoting the efficient translation of mRNAs. It shows enriched expression in the testis as well as notable levels in immune cells and possibly in other tissues. Its involvement in the recognition and binding of N6-methyladenosine (m6A)-modified RNA substrates makes YTHDC2 a significant factor in RNA processing.

      Biological function summary

      YTHDC2 contributes to the regulation of mRNA stability and turnover within the cell. It may operate as part of a complex involved in post-transcriptional control. Through these actions YTHDC2 influences processes such as meiosis and the differentiation of germ cells supported by its association with m6A reader proteins. This association plays a role in the recognition of specific m6A marks critical for RNA dynamics.

      Pathways

      YTHDC2 integrates into the m6A-dependent RNA decay and translation machinery. One important pathway involving this protein is the m6A-mediated gene expression regulation pathway. Within this context YTHDC2 interacts closely with other m6A machinery components such as METTL3 and bridges mRNA modification with subsequent cellular outcomes. Another related pathway is spermatogenesis where the precise control by YTHDC2 is necessary for successful meiosis via interaction with various meiotic proteins.

      Associated diseases and disorders

      Aberrant regulation or expression of YTHDC2 links to infertility as proper expression is necessary for normal germ cell development. This condition involves its association with other reproductive proteins such as those functioning in meiosis. Disorders in mRNA metabolism potentially leading to cancer might also implicate YTHDC2 due to its broad role in regulating mRNA stability and translation highlighting the need to understand its interactions with tumor-related pathways.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      11 product images

      • Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        YTHDC2 was immunoprecipitated from 0.35 mg of HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab220160 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220160 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

        Lane 1: HeLa whole cell lysate 10 μ (Input).
        Lane 2: ab220160IP in HeLa whole cell lysate.
        Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab220160 in HeLa whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.
        Exposure time: 30 seconds.

        All lanes: Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Predicted band size: 160 kDa

        Observed band size: 160 kDa

      • Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Blocking and dilution bufer: 5% NFDM/TBST.

        The wild-type and YTHDC2 knockout cell lysates were kindly provided by an anonymous collaborator.

        ab220160 was shown to specifically react with YTHDC2 in wild-type mESC cells as signal was lost in YTHDC2 knockout cells. Wild-type and YTHDC2 knockout samples were subjected to SDS-PAGE. ab220160 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160) at 1/1000 dilution

        Lane 1: Wild-type mESC (mouse embryo stem cell) whole cell lysate at 40 µg

        Lane 2: YTHDC2 knockout mESC whole cell lysate at 40 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 160 kDa

        Observed band size: 160 kDa

        Exposure time: 3min

        Blocking and dilution bufer: 5% NFDM/TBST.

        The wild-type and YTHDC2 knockout cell lysates were kindly provided by an anonymous collaborator.

        ab220160 was shown to specifically react with YTHDC2 in wild-type mESC cells as signal was lost in YTHDC2 knockout cells. Wild-type and YTHDC2 knockout samples were subjected to SDS-PAGE. ab220160 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

      • Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure times.

        Lane 1: 3 minutes; Lanes 2-3: 37 seconds; Lane 4: 3 minutes; Lane 5: 37 seconds.

        All lanes: Western blot - Anti-YTHDC2 antibody [EPR21820-49] (ab220160) at 1/1000 dilution

        Lane 1: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

        Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

        Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

        Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

        Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 160 kDa

        Observed band size: 160 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat testis (PMID:28380054). Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse testis (PMID:28380054). Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human testis (PMID:28380054). Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Flow Cytometry (Intracellular) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling YTHDC2 with ab220160 at 1/600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

      • Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on mouse testis. Nuclear counterstain: DAPI.

      • Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Negative control: (PMID: 29087293)

        Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on mouse heart. Nuclear counterstain: DAPI.

      • Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on rat testis. Nuclear counterstain: DAPI.

      • Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160), expandable thumbnail

        Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] (ab220160)

        Negative control: (PMID: 29087293)

        Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on rat heart. Nuclear counterstain: DAPI.

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