Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human testis (PMID : 28380054) Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling YTHDC2 with ab220160 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

• IP

Unknown

Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

YTHDC2 was immunoprecipitated from 0.35 mg of HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab220160 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220160 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μ (Input).
Lane 2 : ab220160IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220160 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

All lanes:

Immunoprecipitation - Anti-YTHDC2 antibody [EPR21820-49] (<a href='/en-us/products/primary-antibodies/ythdc2-antibody-epr21820-49-ab220160'>ab220160</a>)

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse testis (PMID : 28380054) Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

Negative control : (PMID : 29087293)

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on rat heart. Nuclear counterstain : DAPI.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling YTHDC2 with ab220160 at 1/500 dilution (1.19 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat testis (PMID : 28380054) Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised rat testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on rat testis. Nuclear counterstain : DAPI.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse testis tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). Showing cytoplasmic staining on mouse testis. Nuclear counterstain : DAPI.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

Negative control : (PMID : 29087293)

Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilised mouse heart tissue labeling YTHDC2 with ab220160 at 1/50 dilution (11 ug/mL). Followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 ug/mL). No staining observed on mouse heart. Nuclear counterstain : DAPI.

• WB

Supplier Data

Western blot - Anti-YTHDC2 antibody [EPR21820-49] - BSA and Azide free (AB259277)

Blocking and dilution bufer : 5% NFDM/TBST.

The wild-type and YTHDC2 knockout cell lysates were kindly provided by an anonymous collaborator.

ab220160 was shown to specifically react with YTHDC2 in wild-type mESC cells as signal was lost in YTHDC2 knockout cells. Wild-type and YTHDC2 knockout samples were subjected to SDS-PAGE. ab220160 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220160).

All lanes:

Western blot - Anti-YTHDC2 antibody [EPR21820-49] (<a href='/en-us/products/primary-antibodies/ythdc2-antibody-epr21820-49-ab220160'>ab220160</a>) at 1/1000 dilution

Lane 1:

Wild-type mESC (mouse embryo stem cell) whole cell lysate at 40 µg

Lane 2:

YTHDC2 knockout mESC whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 160 kDa

Observed band size: 160 kDa

false

Exposure time: 3min