Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)

Rabbit Recombinant Monoclonal YTHDC2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (AB283277), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Not recommended	Expected	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Tested	Not recommended	Tested
Rat	Expected	Tested	Not recommended	Tested	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information YTHDC2

Function

3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells (PubMed:26318451, PubMed:29033321, PubMed:29970596). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability (PubMed:26318451, PubMed:29033321). Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (By similarity). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease (PubMed:29033321). Required for both spermatogenesis and oogenesis (By similarity).

Alternative names

3'-5' RNA helicase YTHDC2, YTH domain-containing protein 2, hYTHDC2, YTHDC2

Recommended products

Rabbit Recombinant Monoclonal YTHDC2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24278-9
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab283277 is the carrier-free version of Anti-YTHDC2 antibody [EPR24278-9] ab271139.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

YTHDC2 also known as YT521-B homology domain containing 2 functions as an RNA helicase with a molecular mass of approximately 160 kDa. This protein modulates RNA metabolism by promoting the efficient translation of mRNAs. It shows enriched expression in the testis as well as notable levels in immune cells and possibly in other tissues. Its involvement in the recognition and binding of N6-methyladenosine (m6A)-modified RNA substrates makes YTHDC2 a significant factor in RNA processing.

Biological function summary

YTHDC2 contributes to the regulation of mRNA stability and turnover within the cell. It may operate as part of a complex involved in post-transcriptional control. Through these actions YTHDC2 influences processes such as meiosis and the differentiation of germ cells supported by its association with m6A reader proteins. This association plays a role in the recognition of specific m6A marks critical for RNA dynamics.

Pathways

YTHDC2 integrates into the m6A-dependent RNA decay and translation machinery. One important pathway involving this protein is the m6A-mediated gene expression regulation pathway. Within this context YTHDC2 interacts closely with other m6A machinery components such as METTL3 and bridges mRNA modification with subsequent cellular outcomes. Another related pathway is spermatogenesis where the precise control by YTHDC2 is necessary for successful meiosis via interaction with various meiotic proteins.

Associated diseases and disorders

Aberrant regulation or expression of YTHDC2 links to infertility as proper expression is necessary for normal germ cell development. This condition involves its association with other reproductive proteins such as those functioning in meiosis. Disorders in mRNA metabolism potentially leading to cancer might also implicate YTHDC2 due to its broad role in regulating mRNA stability and translation highlighting the need to understand its interactions with tumor-related pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunoprecipitation - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
YTHDC2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: Anti-YTHDC2 antibody [EPR24278-9] ab271139 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-YTHDC2 antibody [EPR24278-9] ab271139 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.
Lysates were made freshly and used in IP test immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-YTHDC2 antibody [EPR24278-9] (Anti-YTHDC2 antibody [EPR24278-9] ab271139)
Predicted band size: 160 kDa
Observed band size: 160 kDa
Western blot - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: Lane 1-2, 4-5: 37 seconds. Lane 3: 3 minutes.
All lanes: Western blot - Anti-YTHDC2 antibody [EPR24278-9] (Anti-YTHDC2 antibody [EPR24278-9] ab271139) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
Immunoprecipitation - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
YTHDC2 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10μg
Lane 2: Anti-YTHDC2 antibody [EPR24278-9] ab271139 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-YTHDC2 antibody [EPR24278-9] ab271139 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 67 seconds.
Lysates were made freshly and used in IP test immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-YTHDC2 antibody [EPR24278-9] (Anti-YTHDC2 antibody [EPR24278-9] ab271139)
Predicted band size: 160 kDa
Observed band size: 160 kDa
Western blot - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW & expression profile are consistent with what has been described in the literature (PMID: 29799838, PMID: 29087293).
Low expression: heart (PMID: 29799838, PMID: 29087293).
Exposure time: 15 seconds.
All lanes: Western blot - Anti-YTHDC2 antibody [EPR24278-9] (Anti-YTHDC2 antibody [EPR24278-9] ab271139) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
Western blot - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW & expression profile are consistent with what has been described in the literature (PMID: 29799838, PMID: 29087293).
Low expression: heart (PMID: 29799838, PMID: 29087293).
Exposure time: 114 seconds.
All lanes: Western blot - Anti-YTHDC2 antibody [EPR24278-9] (Anti-YTHDC2 antibody [EPR24278-9] ab271139) at 1/1000 dilution
Lane 1: Rat testis tissue lysate at 20 µg
Lane 2: Rat heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/2000 (0.285 μg/mL) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse testis (PMID: 28809393). The section was incubated with Anti-YTHDC2 antibody [EPR24278-9] ab271139 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/2000 (0.285 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat testis. The section was incubated with Anti-YTHDC2 antibody [EPR24278-9] ab271139 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/2000 (0.285 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat testis. The section was incubated with Anti-YTHDC2 antibody [EPR24278-9] ab271139 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/2000 (0.285 μg/mL) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with Anti-YTHDC2 antibody [EPR24278-9] ab271139 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh) tissue labeling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/50 (11.4 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on mouse testis is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/50 (11.4 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
No staining on mouse heart
Negative control: (PMID: 29087293)
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis (fresh) tissue labeling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/50 (11.4 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat testis is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-YTHDC2 antibody [EPR24278-9] - BSA and Azide free (ab283277)
This data was developed using Anti-YTHDC2 antibody [EPR24278-9] ab271139, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling YTHDC2 with Anti-YTHDC2 antibody [EPR24278-9] ab271139 at 1/50 (11.4 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
No staining on rat heart
Negative control: (PMID: 29087293)
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.