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AB252547

Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free

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Rabbit Recombinant Monoclonal YTHDF1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Rat, Mouse samples.

View Alternative Names

C20orf21, YTHDF1, YTH domain-containing family protein 1, DF1, Dermatomyositis associated with cancer putative autoantigen 1, DACA-1

3 Images
Flow Cytometry (Intracellular) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cell line labeling YTHDF1 with ab252346 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252346).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling YTHDF1 with ab252346 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrum (PMID : 29103884; 26046440) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252346).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF1 antibody [EPR22349-16] - BSA and Azide free (AB252547)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling YTHDF1 with ab252346 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum (PMID : 29103884; 26046440) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252346).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22349-16

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab252547 is the carrier-free version of ab252346.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YTHDF1 also known as YTH domain family protein 1 is a reader protein of N6-methyladenosine (m6A) a common RNA modification. Its mass is approximately 64 kDa. YTHDF1 binds to m6A-modified mRNA and enhances its translation efficiency by recruiting ribosomes. This protein is present in various tissues showing higher expression in brain spleen and lymphoid tissues suggesting a role in development and immunity.
Biological function summary

YTHDF1 affects mRNA stability and translation providing a mechanism for regulating protein synthesis. It operates as part of a complex with other m6A readers like YTHDF2 and YTHDF3 which determine mRNA fate. By promoting translation YTHDF1 impacts cellular responses and adaptation to environmental changes especially during stress and division.

Pathways

YTHDF1 integrates into the mRNA surveillance pathway and translation regulation. It interacts closely with other components such as eukaryotic initiation factors (eIFs) to modulate protein synthesis dynamics. This allows cells to rapidly adjust protein levels in response to cues and maintain homeostasis. Its role is critical in link to ribosomal function and stability of gene expression networks.

YTHDF1 has connections to cancer and neurological disorders. In cancer abnormal YTHDF1 expression associates with tumor progression and metastasis and interacts with proteins like METTL3 involved in m6A deposition. In neurological disorders dysregulation of YTHDF1 influences mRNA translation tied to cognitive functions possibly interacting with neurotransmitter pathways. Understanding these roles shows potential for YTHDF1 as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability (PubMed : 24284625, PubMed : 26318451, PubMed : 32492408, PubMed : 39900921). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed : 24284625, PubMed : 32492408). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex (PubMed : 32492408). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed : 28106072, PubMed : 32492408). Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues : polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (PubMed : 31292544, PubMed : 31388144, PubMed : 32451507). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (PubMed : 31292544).
See full target information YTHDF1
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Product promise

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For full details, please see our Terms & Conditions

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