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Rabbit Recombinant Monoclonal YTHDF1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IP, WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749), expandable thumbnail
  • Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749), expandable thumbnail
  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749), expandable thumbnail
  • Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Tested
Not recommended
Expected
Tested
Not recommended

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability (PubMed:24284625, PubMed:26318451, PubMed:32492408). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:24284625, PubMed:32492408). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex (PubMed:32492408). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed:28106072, PubMed:32492408). Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (PubMed:31292544, PubMed:31388144, PubMed:32451507). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (PubMed:31292544).

Additional Targets

YTHDF2, YTHDF3

Alternative names

C20orf21, YTHDF1, YTH domain-containing family protein 1, DF1, Dermatomyositis associated with cancer putative autoantigen 1, DACA-1

Recommended products

Rabbit Recombinant Monoclonal YTHDF1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IP, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26183-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YTHDF1 YTHDF2 and YTHDF3 also known as YTH domain family proteins are key readers of N6-methyladenosine (m6A) RNA modifications. These proteins each weigh around 63-68 kDa and show expression across various human tissues with notable activity in the brain and liver. YTHDF proteins bind to m6A-modified RNAs facilitating subsequent RNA metabolism processes such as translation and decay. By recognizing m6A marks YTHDF1 2 and 3 influence RNA fate therefore playing an important role in post-transcriptional gene regulation.

Biological function summary

YTHDF proteins facilitate the dynamic control of mRNA stability and translation efficiency. They are known to interact with each other forming complexes that further strengthen their functional impact on RNA targets. In particular YTHDF1 enhances translation by recruiting ribosomes to m6A-modified mRNAs. YTHDF2 primarily targets mRNA for degradation therefore reducing mRNA stability. Meanwhile YTHDF3 supports both YTHDF1-mediated translation and YTHDF2-mediated degradation acting as a versatile mediator in RNA regulation.

Pathways

These proteins play important roles in cellular homeostasis and stress responses. The m6A modification pathway involves collaboration with proteins such as METTL3 and FTO which add and remove m6A marks respectively. The interaction of YTHDF proteins within this pathway allows cells to quickly respond to stimuli by adjusting protein production levels. Their functions align with signaling pathways like the mTOR pathway where they influence translation rates in response to nutrient availability.

Associated diseases and disorders

YTHDF proteins are linked to cancers and neurological conditions. Dysregulation of these proteins associates with tumorigenesis particularly through interactions with oncogenic proteins such as c-Myc. Abnormal YTHDF function also connects to neurodegenerative disorders where alterations in mRNA metabolism lead to neural dysfunction. The ability of YTHDF proteins to bind specific m6A-modified mRNAs makes them targets for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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