AB290749

Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal YTHDF1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IP, WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

C20orf21, YTHDF1, YTH domain-containing family protein 1, DF1, Dermatomyositis associated with cancer putative autoantigen 1, DACA-1, YTH domain-containing family protein 3, DF3, YTHDF3, HGRG8, YTHDF2, YTH domain-containing family protein 2, DF2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/50 (10.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/mL) dilution (Green). Confocal image showing mostly cytoplasmic staining in Hela cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2ug/mL) dilution.

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290749, the same antibody clone in a different buffer formulation.

YTHDF1 + YTHDF2 + YTHDF3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug ab290734 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using anti YTHDF1 + YTHDF2 + YTHDF3 antibody (ab290734) at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : anti YTHDF1 + YTHDF2 + YTHDF3 antibody (ab290734) IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab290734 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (<a href='/en-us/products/primary-antibodies/ythdf1-ythdf3-ythdf2-antibody-epr26183-69-ab290734'>ab290734</a>)

false

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized NIH/3T3 (mouse embryonic fibroblast) cells lebelling YTHDF1 + YTHDF2 + YTHDF3 with ab290734 at 1/50 (10.04 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/mL) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2ug/mL) dilution.

Supplier Data

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

YTHDF1 + YTHDF2 + YTHDF3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab290734 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab290734 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

Lane 2 : ab290734 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab290734 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (<a href='/en-us/products/primary-antibodies/ythdf1-ythdf3-ythdf2-antibody-epr26183-69-ab290734'>ab290734</a>)

false

Lab

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Blocking / Diluent buffer and concentration : 5% NFDM/TBST.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

All lanes:

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (<a href='/en-us/products/primary-antibodies/ythdf1-ythdf3-ythdf2-antibody-epr26183-69-ab290734'>ab290734</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate. at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate. at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 61 kDa

false

Exposure time: 70s

Lab

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

This data was developed using ab290734, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (<a href='/en-us/products/primary-antibodies/ythdf1-ythdf3-ythdf2-antibody-epr26183-69-ab290734'>ab290734</a>) at 1/1000 dilution

Lane 1:

His-tagged human YTHDF1 recombinant protein 10ng

Lane 2:

His-tagged human YTHDF2 recombinant protein 10ng

Lane 3:

His-tagged human YTHDF3 recombinant protein 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 61 kDa

false

Exposure time: 70s

Unconjugated

Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26183-69

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

IP, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YTHDF1 YTHDF2 and YTHDF3 also known as YTH domain family proteins are key readers of N6-methyladenosine (m6A) RNA modifications. These proteins each weigh around 63-68 kDa and show expression across various human tissues with notable activity in the brain and liver. YTHDF proteins bind to m6A-modified RNAs facilitating subsequent RNA metabolism processes such as translation and decay. By recognizing m6A marks YTHDF1 2 and 3 influence RNA fate therefore playing an important role in post-transcriptional gene regulation.

Biological function summary

YTHDF proteins facilitate the dynamic control of mRNA stability and translation efficiency. They are known to interact with each other forming complexes that further strengthen their functional impact on RNA targets. In particular YTHDF1 enhances translation by recruiting ribosomes to m6A-modified mRNAs. YTHDF2 primarily targets mRNA for degradation therefore reducing mRNA stability. Meanwhile YTHDF3 supports both YTHDF1-mediated translation and YTHDF2-mediated degradation acting as a versatile mediator in RNA regulation.

Pathways

These proteins play important roles in cellular homeostasis and stress responses. The m6A modification pathway involves collaboration with proteins such as METTL3 and FTO which add and remove m6A marks respectively. The interaction of YTHDF proteins within this pathway allows cells to quickly respond to stimuli by adjusting protein production levels. Their functions align with signaling pathways like the mTOR pathway where they influence translation rates in response to nutrient availability.

YTHDF proteins are linked to cancers and neurological conditions. Dysregulation of these proteins associates with tumorigenesis particularly through interactions with oncogenic proteins such as c-Myc. Abnormal YTHDF function also connects to neurodegenerative disorders where alterations in mRNA metabolism lead to neural dysfunction. The ability of YTHDF proteins to bind specific m6A-modified mRNAs makes them targets for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability (PubMed : 24284625, PubMed : 26318451, PubMed : 32492408, PubMed : 39900921). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed : 24284625, PubMed : 32492408). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex (PubMed : 32492408). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed : 28106072, PubMed : 32492408). Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues : polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (PubMed : 31292544, PubMed : 31388144, PubMed : 32451507). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (PubMed : 31292544).

See full target information YTHDF1

Additional targets

YTHDF2,YTHDF3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:6217272 PubMed40568348

2025

WTAP Accelerates Exhaustion of CD8 T Cells and Progression of Hepatocellular Carcinoma by Promoting m6A Modification and Translation of PD1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Shunle Li,Hua Li,Bingli Liu,Zimu Wang,Huanqin Lei,Yuting Li,Lijuan Jia,Junhui Li,Hongwei Lu,Meng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Immunoprecipitation - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Western blot - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free (AB290749)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise