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Anti-YTHDF2 antibody [EPR20318] (ab220163) is a rabbit monoclonal antibody that is used to detect YTHDF2 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with YTHDF2 knockout cell line validation


Images

Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (AB220163), expandable thumbnail
  • Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163), expandable thumbnail
  • Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163), expandable thumbnail
  • Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163), expandable thumbnail
  • Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFIHC-PIPWB
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Expected
Tested
Rat
Not recommended
Not recommended
Not recommended
Expected
Tested
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Rat, Mouse, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability (PubMed:24284625, PubMed:26046440, PubMed:26318451, PubMed:32492408). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661, PubMed:32492408). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context (PubMed:24284625, PubMed:26046440, PubMed:27558897, PubMed:30930054, PubMed:32492408). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed:28106072, PubMed:32492408). M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex (PubMed:30930054). Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs (PubMed:27558897). Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (By similarity). Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases (By similarity). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation (PubMed:30065315). Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets (By similarity). Inhibits neural specification of induced pluripotent stem cells by binding to methylated neural-specific mRNAs and promoting their degradation, thereby restraining neural differentiation (PubMed:32169943). Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts (PubMed:30428350). Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation (PubMed:30559377). May also act as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (PubMed:26458103). Regulates mitotic entry by promoting the phase-specific m6A-dependent degradation of WEE1 transcripts (PubMed:32267835). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (PubMed:31292544, PubMed:31388144, PubMed:31642031, PubMed:32451507). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (PubMed:31292544). May also recognize and bind RNAs modified by C5-methylcytosine (m5C) and act as a regulator of rRNA processing (PubMed:31815440). (Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs (PubMed:29447282). (Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells) (PubMed:29659627). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs (PubMed:29659627).

Alternative names

HGRG8, YTHDF2, YTH domain-containing family protein 2, DF2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2

Recommended products

Anti-YTHDF2 antibody [EPR20318] (ab220163) is a rabbit monoclonal antibody that is used to detect YTHDF2 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with YTHDF2 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20318
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-YTHDF2 antibody [EPR20318] (ab220163) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), in Human, Mouse, Rat samples.

What is the molecular weight of YTHDF2?


Anti-YTHDF2 [EPR20318] (ab220163) specifically detects a band for YTHDF2 (UniProt: Q9Y5A9) at a molecular weight of 62kDa.

Recommended positive controls


WB: Wild-type mESC, HEK-293T, HT-1080 treated with 10µM MG-132 for 4 hours, K562, HEK-293 and HeLa whole cell lysate. Human brain, mouse and rat testis tissue lysate; GST-tagged human YTHDF2 recombinant protein.IP: HepG2 cells

Trusted by the scientific community


Anti-YTHDF2 [EPR20318] (ab220163) was first used in a scientific publication in 2018 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-YTHDF2 antibody [EPR20318] (ab220163) has been confirmed by Western blot testing in YTHDF2 Knockout mESC cells.



Other related products


We have a range of other formats of antibody clone [EPR20318] also available for your convenience:
ab220163, Carrier free - Anti-YTHDF2 antibody [EPR20318] - BSA and Azide free ab245129



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YTHDF2 also known as YTH domain family member 2 mechanically functions as a reader of N6-methyladenosine (m6A)-modified RNA. YTHDF2 binds to these m6A-modified sites on messenger RNA leading to degradation of these transcripts. This protein acts in the cytoplasm and has a molecular weight of approximately 65 kDa. YTHDF2 is highly expressed in various tissues including in the brain liver and reproductive organs.

Biological function summary

YTHDF2 plays a critical role in regulating mRNA stability and decay impacting gene expression dynamically. It acts as an important regulator in processes such as cell differentiation and response to stress. YTHDF2 does not seem to form a complex but interacts with other proteins to fulfill its regulatory functions. Importantly these interactions help manage the cellular RNA cycle maintaining proper cellular physiology.

Pathways

YTHDF2 integrates into the mRNA surveillance pathway and influences processes such as the circadian rhythm and immune response. It works with proteins such as DCP1/2 which are part of the mRNA decay pathway to ensure rapid turnover of target mRNAs. YTHDF2's control over transcript degradation connects it with RNA-processing machinery impacting pathways reliant on precise proteomic modulation.

Associated diseases and disorders

Abnormal YTHDF2 function can contribute to cancer and metabolic disorders. In cancer its interaction with tumor suppressor proteins like p53 alters transcript levels important for cancer cell survival. In metabolic disorders YTHDF2's effect on mRNA turnover can influence pathways involving insulin secretion. YTHDF2's involvement in these diseases suggests it as a potential target for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    ab220163 Immunoprecipitating YTHDF2 in human HepG2 whole cell lysate. 10 μg of cell lysate was incubated with primary antibody (1/30). For western blotting ab220163 (1/1000) was used to confirm successful immunoprecipitation. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at (1/5000). For lane 3 Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used instead of ab220163.

    In panel A, the lysate was stored at -80oC prior to the IP and incubation with the primary antibody was carried out overnight. In panel B, lysate was freshly made and used in the IP test immediately to minimize protein degradation. The incubation time was also shortened from overnight to 2h.

    All lanes: Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

    Exposure time: 3s

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    The wild-type and YTHDF2 knockout cell lysates were kindly provided by an anonymous collaborator.

    ab220163 was shown to specifically react with YTHDF2 in wild-type mESC cells as signal was lost in YTHDF2 knockout cells. Wild-type and YTHDF2 knockout samples were subjected to SDS-PAGE. ab220163 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    All lanes: Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution

    Lane 1: Wild-type mESC (mouse embryo stem cell) whole cell lysate at 20 µg

    Lane 2: YTHDF2 knockout mESC whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

    Exposure time: 48s

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution

    Lane 1: Untreated HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HT-1080 treated with 10μM MG-132 (MG-132, proteasome inhibitor ab141003) for 4 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

    Exposure time: 103s

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 3 minutes; Lane 2: 92 seconds; Lane 3: 37 seconds; Lane 4: 8 seconds; Lane 5: 15 seconds.

    All lanes: Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 10 µg

    Lane 2: Mouse testis tissue lysate at 10 µg

    Lane 3: Rat testis tissue lysate at 10 µg

    Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

    Lane 5: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

    Secondary

    Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 2 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    YTHDF2 Western blot staining using rabbit Anti-YTHDF2 antibody

    The YTHDF recombinant proteins were kindly provided by an anonymous collaborator.

    All lanes: Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/1000 dilution

    Lane 1: GST-tagged human YTHDF1 recombinant protein, 20ng

    Lane 2: GST-tagged human YTHDF2 recombinant protein, 20ng

    Lane 3: GST-tagged human YTHDF3 recombinant protein, 20ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa

    Exposure time: 3s

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163) at 1/5000 dilution

    Lane 1: Untreated HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HT-1080 treated with 10μM MG-132 (MG-132, proteasome inhibitor ab141003) for 4 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 103s

  • Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-YTHDF2 antibody [EPR20318] (ab220163)

    YTHDF2 western blot using anti-YTHDF2 antibody [EPR20318] ab220163. Publication image and figure legend from Jin, D., Guo, J., et al., 2020, Mol Cancer, PubMed 32106857.


    ab220163 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab220163 please see the product overview.

    YTHDF1 and YTHDF2 competitively interacted with YTHDF3 in an m6A- independent manner to regulate YAP expression. (a) The diagram of that YTHDF1 and YTHDF2 competitively interacted with YTHDF3 to regulate YAP expression in NSCLC. (b, c) The interaction between YTHDF1/2/3 and YAP mRNA was determined by RIP assay. (d) The interactions between YTHDF1/YTHDF2 and the mRNA of YAP were increased when YTHDF3 is existed determined by RNA pulldown assay. (e) Western blot analysis indicated that FTO and ALKBH5 were only in nuclear fractions but YTHDF1/2/3 were only in cytoplasm fractions in A549 cells. (f, g) Immunofluorescent staining showed that ALKBH5 WT/KD were only in nuclear fractions (f) but YTHDF1/2/3 were only in cytoplasm fractions (g) in A549 cells. (h) Co-IPs performed using lysates collected from A549 cells with immunoprecipitation by either YTHDF1, YTHDF2 or YTHDF3 antibodies. (i) The protein level of YTHDF3 was analyzed in lysates collected from either YTHDF1 or YTHDF2 transfected A549 and H1299 cells determined by Co-IPs assays by immunoprecipitation with YTHDF2 or YTHDF1 antibodies, respectively. (j, k) The interactions between YTHDF1/2/3 and YAP mRNA were detected in A549 and H1299 cells with transfection with indicated genes determined by Co-IP assay using the MS2 coat protein system. (l) The interactions between YTHDF1/2 and YAP mRNA was determined by RIP assay in A549 and H1299 cells with transfection of indicated genes. (m) The m6A levels of YAP were analyzed by in A549 and H1299 cells with transfection into indicated genes. (n) The protein level of YTHDF3 was detected in lysates collected from A549 cells determined by Co-IP assay with immunoprecipitation with either YTHDF1 or YTHDF2 antibodies. Results were presented as mean ± SD of three independent experiments. *P < 0.05 or **P < 0.01 indicates a significant difference between the indicated groups. ns, not significant

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