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AB317173

Anti-YTHDF3 antibody [HL2235]

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Rabbit Recombinant Monoclonal YTHDF3 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human YTHDF3.

View Alternative Names

YTH domain-containing family protein 3, DF3, YTHDF3

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-YTHDF3 antibody [HL2235] (AB317173)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-YTHDF3 antibody [HL2235] (AB317173)

ab317173 detects YTHDF3 protein at cytoplasm by immunofluorescent analysis. Sample : HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : YTHDF3 stained by ab317173 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [HL2235] (AB317173)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [HL2235] (AB317173)

ab317173 detects YTHDF3 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat brain. YTHDF3 stained by ab317173 diluted at 1 : 200. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [HL2235] (AB317173)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDF3 antibody [HL2235] (AB317173)

ab317173 detects YTHDF3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. YTHDF3 stained by ab317173 diluted at 1 : 200. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-YTHDF3 antibody [HL2235] (AB317173)
  • WB

Lab

Western blot - Anti-YTHDF3 antibody [HL2235] (AB317173)

Western blot : Anti-YTHDF3 antibody [HL2235] ab317173 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70 kDa in Wild-type PC-3 cell lysates with no signal observed at this size in YTHDF3 knockout PC-3 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-YTHDF3 antibody [HL2235] (ab317173) at 1/2000 dilution

Lane 1:

Wild-type PC-3 cell lysates at 20 µg

Lane 2:

Western blot - Human YTHDF3 knockout PC-3 cell line (<a href='/en-us/products/cell-lines/human-ythdf3-knockout-pc-3-cell-line-ab290587'>ab290587</a>) at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Lane 4:

HT-1080 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 70 kDa

false

Western blot - Anti-YTHDF3 antibody [HL2235] (AB317173)
  • WB

Supplier Data

Western blot - Anti-YTHDF3 antibody [HL2235] (AB317173)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317173 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-YTHDF3 antibody [HL2235] (ab317173) at 1/2000 dilution

Lane 1:

Huh-7 whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2235

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human YTHDF3.

Q7Z739

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The YTH domain family protein 3 (YTHDF3) is an m6A reader protein known also as YTH3 or YTHDF2-like protein 3. Its molecular weight is approximately 64 kDa. YTHDF3 plays a role in the recognition of N6-methyladenosine (m6A)-modified RNA an important post-transcriptional modification. It commonly expresses in various tissues including the brain liver and kidney indicating its involvement in multiple cellular processes. YTHDF3 binds to m6A-modified mRNA influencing the stability and translation of these molecules and interacts with other members of the YTH family.
Biological function summary

YTHDF3 facilitates the process of mRNA metabolism by participating in the regulation of mRNA translation and degradation. It functions as part of a dynamic complex with YTHDF1 and YTHDF2 coordinating the fate of m6A-modified mRNAs. YTHDF3 supports mRNA decay in synergy with YTHDF2 and enhances translation efficiency in cooperation with YTHDF1. This involvement in regulating gene expression at the post-transcriptional level equips cells to respond rapidly to various stimuli aligning gene expression with cellular needs.

Pathways

YTHDF3 significantly impacts RNA processing and metabolism pathways. YTHDF3 together with YTHDF1 and YTHDF2 modulates the m6A-mediated mRNA decay pathway. It holds functional significance in the cellular response to stress and adaptations in energy metabolism. YTHDF3 interacts closely with proteins such as IGF2BP1 alongside the YTHDF proteins which are critical in maintaining RNA stability and translation thereby illustrating its role across significant cellular pathways.

YTHDF3 has implications in cancer and neurodegenerative diseases. High YTHDF3 expression associates with various cancer types including gliomas and breast cancer where it can influence tumor growth and metastasis. In neurodegenerative diseases such as Alzheimer's disease YTHDF3 interacts with proteins like YTHDF1 and YTHDF2 affecting RNA metabolism in neurons contributing potentially to the pathology of these disorders. Through these interactions YTHDF3 could impact disease progression and outcomes making it a target of interest in therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability (PubMed : 28106072, PubMed : 28106076, PubMed : 28281539, PubMed : 32492408). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed : 22575960, PubMed : 24284625, PubMed : 28106072, PubMed : 28281539, PubMed : 32492408). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex or PAN3 (PubMed : 32492408). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed : 28106072, PubMed : 28106076, PubMed : 32492408). Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression : acts by binding to FOXO3 mRNAs (By similarity). Binds to FOXO3 mRNAs independently of METTL3-mediated m6A modification (By similarity). Can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation (PubMed : 28106072). Recognizes and binds m6A-containing circular RNAs (circRNAs); circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons (PubMed : 28281539). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues : polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (PubMed : 31292544, PubMed : 31388144, PubMed : 32451507). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (PubMed : 31292544). May also recognize and bind N1-methyladenosine (m1A)-containing mRNAs : inhibits trophoblast invasion by binding to m1A-methylated transcripts of IGF1R, promoting their degradation (PubMed : 32194978).. Has some antiviral activity against HIV-1 virus : incorporated into HIV-1 particles in a nucleocapsid-dependent manner and reduces viral infectivity in the next cycle of infection (PubMed : 32053707). May interfere with this early step of the viral life cycle by binding to N6-methyladenosine (m6A) modified sites on the HIV-1 RNA genome (PubMed : 32053707).
See full target information YTHDF3
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Product promise

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