Rabbit Recombinant Monoclonal YWHAE antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (AB239903), expandable thumbnail](https://content.abcam.com/products/images/ywhae-antibody-epr3918-bsa-and-azide-free-ab239903--flow-cytometry-intracellular-img114770.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (AB239903)")
![Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (AB239903), expandable thumbnail](https://content.abcam.com/products/images/ywhae-antibody-epr3918-bsa-and-azide-free-ab239903--immunocytochemistry-immunofluorescence-img51639.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (AB239903)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested | Tested |
| Mouse | Not recommended | Expected | Not recommended | Predicted | Predicted |
| Rat | Not recommended | Expected | Not recommended | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).
Alternative names
14-3-3 protein epsilon, 14-3-3E, YWHAE
Recommended products
Rabbit Recombinant Monoclonal YWHAE antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Alternative names
14-3-3 protein epsilon, 14-3-3E, YWHAE
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3918
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239903 is the carrier-free version of Anti-YWHAE antibody [EPR3918] ab92311.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The YWHAE protein also known as 14-3-3E acts as a regulatory molecule by binding to various phosphoserine-containing proteins. It plays key roles in cellular signaling and exists as a dimer. YWHAE has a molecular mass of around 29 kDa and is widely expressed in tissues including the brain heart and skeletal muscle. Its interactions often rely on its abundant presence in various cell types showing its versatility.
- Biological function summary
YWHAE protein interacts with several enzymes and receptors forming complexes that affect their functional states. It is a member of the 14-3-3 family which facilitates signal transduction by binding to phosphorylated serine/threonine residues. This protein influences processes like cell cycle control apoptosis and stress responses emphasizing its significant roles in maintaining cellular homeostasis.
- Pathways
YWHAE plays significant roles in the Akt and MAPK pathways. In the Akt pathway YWHAE partners with proteins like BAD and FOXO which are important in controlling apoptosis and cell survival. In the MAPK pathway it regulates multiple proteins including Raf and MEK impacting cell growth and differentiation. Understanding YWHAE's interactions in these pathways highlights its influence in critical signaling events.
- Associated diseases and disorders
YWHAE has been tied to schizophrenia and neurodevelopmental disorders. In schizophrenia it interacts with proteins like DISC1 affecting neuronal migration and synaptic function. In neurodevelopmental disorders alterations in YWHAE can lead to compromised signaling that disrupts normal brain function. These connections highlight the importance of YWHAE in maintaining neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903), expandable thumbnail](https://content.abcam.com/products/images/ywhae-antibody-epr3918-bsa-and-azide-free-ab239903--flow-cytometry-intracellular-img114770.jpg "Flow Cytometry (Intracellular) - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903)")
Flow Cytometry (Intracellular) - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903)
Overlay histogram showing SH-SY5Y cells stained with Anti-YWHAE antibody [EPR3918] ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-YWHAE antibody [EPR3918] ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YWHAE antibody [EPR3918] ab92311).
![Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903), expandable thumbnail](https://content.abcam.com/products/images/ywhae-antibody-epr3918-bsa-and-azide-free-ab239903--immunocytochemistry-immunofluorescence-img51639.jpg "Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903)")
Immunocytochemistry/ Immunofluorescence - Anti-YWHAE antibody [EPR3918] - BSA and Azide free (ab239903)
Anti-YWHAE antibody [EPR3918] ab92311 at 1/100 dilution staining YWHAE in HeLa cells by Immunofluorescence.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-YWHAE antibody [EPR3918] ab92311).
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