Rabbit Polyclonal YY1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human YY1 aa 50-100.
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- Journal of microbiology and biotechnology 32:1406-14152022Silencing YY1 Alleviates Ox-LDL-Induced Inflammation and Lipid Accumulation in Macrophages through Regulation of PCSK9/ LDLR Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengyao Qian,Jianping ZhaoPubMed 36330745
- Frontiers in genetics 13:9454142022Identification of CXCL10 and CXCL11 as the candidate genes involving the development of colitis-associated colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCan Lu,Xiaopeng Zhang,Yang Luo,Jingang Huang,Minhao YuPubMed 36003333
- Cells 10:2021The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicoletta Bianchi,Federica Brugnoli,Silvia Grassilli,Karine Bourgeois,Jeffrey W Keillor,Carlo M Bergamini,Gianluca Aguiari,Stefano Volinia,Valeria BertagnoloPubMed 34831282
- Non-coding RNA 7:2021Inhibition of the lncRNA Coded within Transglutaminase 2 Gene Impacts Several Relevant Networks in MCF-7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlo M Bergamini,Chiara Vischioni,Gianluca Aguiari,Carmen Grandi,Anna Terrazzan,Stefano Volinia,Nicoletta Bianchi,Cristian TaccioliPubMed 34449674
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human YY1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P25490
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence (PubMed:1655281). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed (PubMed:11158321). Involved in DNA repair (PubMed:18026119, PubMed:28575647). In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation (PubMed:28575647). Recruits the PR-DUB complex to specific gene-regulatory regions (PubMed:20805357). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.
Alternative names
INO80S, YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1
Recommended products
Rabbit Polyclonal YY1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human YY1 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human YY1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P25490
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245365 was affinity purified using an epitope specific to YY1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YY1 also known as Yin Yang 1 is a transcription factor that regulates gene expression by binding to specific DNA sequences. It is a protein with a molecular mass of approximately 68 kDa and is ubiquitously expressed in many tissues including liver lung and kidney. Functionally YY1 can both activate and repress transcription making it versatile in regulating gene activity. It interacts with several co-factors and other proteins influencing chromatin structure and gene expression.
- Biological function summary
Yin Yang 1 plays an important role in various cellular processes such as cell proliferation differentiation and apoptosis. It is part of a chromatin remodeling complex where it assists in modifying the architecture of chromatin to control gene activity. Due to its ability to bind to diverse DNA motifs YY1 is involved in embryonic development and cellular differentiation. Additionally it interacts with other transcription factors to coordinate the expression of genes critical for cell cycle progression and survival.
- Pathways
The Yin Yang 1 transcription factor is integrally involved in several key signaling pathways. It plays a notable role in the Wnt signaling pathway which is vital for controlling cell growth and differentiation. YY1 interacts with proteins such as β-catenin an important player in the Wnt pathway to mediate its transcriptional effects. Furthermore it also functions within the mTOR signaling pathway associating with proteins like mTOR itself to impact cell metabolism and growth.
- Associated diseases and disorders
Alterations in the expression or function of YY1 have been implicated in several conditions. Dysregulation of YY1 has strong associations with cancer as its interactions with proteins like MYC can lead to abnormal cell proliferation and tumor development. Additionally its role in neuronal differentiation links it with neurological disorders such as Alzheimer's disease. In these contexts YY1's interaction with other transcription factors and regulatory proteins influences disease progression and severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-YY1 antibody (ab245365)
All lanes: Western blot - Anti-YY1 antibody (ab245365) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30s
Immunoprecipitation - Anti-YY1 antibody (ab245365)
YY1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245365 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245365 at 1 μg/ml.
Lane 1: ab245365 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-YY1 antibody (ab245365)
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody (ab245365)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue (left) and mouse teratoma tissue (right) stained for YY1 using ab245365 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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