Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
- RabMAb
- Recombinant
- Lab Essentials
- Advanced Validation
- 20ul selling size
- What is this?
4
(10 Reviews)
|
(76 Publications)
Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) is a rabbit monoclonal antibody detecting YY1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
View Alternative Names
INO80S, YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control : primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
ab109237 staining YY1 in the human cell line HeLa (Human epithelial cell line from cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control : Rabbit monoclonal IgG (Black).
Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution
Lane 1:
Y79 (Human retinoblastoma cell line) cell lysate at 10 µg
Lane 2:
HuT-78 cell lysate at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa
false
- WB
Unknown
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
All lanes:
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution
Lane 1:
Daudi (Human Burkitt's lymphoma cell line) cell lysate at 10 µg
Lane 2:
Y79 (Human retinoblastoma cell line) cell lysate at 10 µg
Lane 3:
HuT-78 cell lysate at 10 µg
Predicted band size: 45 kDa
false
- WB
Lab
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 2:
Daudi (Human Burkitt's lymphoma cell line) cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa
false
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- WB
Lab
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (AB109237)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution
Lane 1:
Mouse heart at 10 µg
Lane 2:
Rat heart at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa
false
Related conjugates and formulations (3)
-
Anti-YY1 antibody [EPR4652] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-YY1 antibody [EPR4652] - Nuclear Marker
-
Anti-YY1 antibody [EPR4652] - BSA and Azide free (Detector)
Reactivity data
Product details
What is this antibody validated in?
Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of YY1?
Anti-YY1 [EPR4652] - Nuclear Loading Control (ab109237) is often used as a loading control in Western blot. It specifically detects a band for YY1 (UniProt: P25490) at a molecular weight of 45kDa.
Trusted by the scientific community
Anti-YY1 [EPR4652] - Nuclear Loading Control (ab109237) was first used in a scientific publication in 2011 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-YY1 [EPR4652] - Nuclear Loading Control (ab109237) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR4652] also available for your convenience: ab109237, Alexa Fluor® 488 - ab199814, Carrier free - ab232573, Carrier free - ab281076
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Yin Yang 1 plays an important role in various cellular processes such as cell proliferation differentiation and apoptosis. It is part of a chromatin remodeling complex where it assists in modifying the architecture of chromatin to control gene activity. Due to its ability to bind to diverse DNA motifs YY1 is involved in embryonic development and cellular differentiation. Additionally it interacts with other transcription factors to coordinate the expression of genes critical for cell cycle progression and survival.
Pathways
The Yin Yang 1 transcription factor is integrally involved in several key signaling pathways. It plays a notable role in the Wnt signaling pathway which is vital for controlling cell growth and differentiation. YY1 interacts with proteins such as Β-catenin an important player in the Wnt pathway to mediate its transcriptional effects. Furthermore it also functions within the mTOR signaling pathway associating with proteins like mTOR itself to impact cell metabolism and growth.
Product protocols
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Target data
Publications (76)
Recent publications for all applications. Explore the full list and refine your search
JCI insight 10: PubMed41059571
2025
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The Journal of clinical investigation 135: PubMed40762953
2025
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Nature communications 16:6261 PubMed40623999
2025
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Nature communications 16:3307 PubMed40204699
2025
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Clinical and experimental medicine 25:99 PubMed40156642
2025
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Cell death & disease 16:144 PubMed40021626
2025
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Nature communications 16:1324 PubMed39900599
2025
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EMBO reports 26:810-835 PubMed39747661
2025
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Blood cancer journal 14:218 PubMed39695118
2024
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Cell death & disease 15:862 PubMed39604408
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com