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Rabbit Recombinant Multiclonal YY1 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human YY1.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (AB313420), expandable thumbnail
  • Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (AB313420), expandable thumbnail
  • Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (AB313420), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human YY1. Database link P25490

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Target data

Function

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression (PubMed:15329343, PubMed:17721549, PubMed:24326773, PubMed:25787250). For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence (PubMed:1655281). Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed (PubMed:11158321). Involved in DNA repair (PubMed:18026119, PubMed:28575647). In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation (PubMed:28575647). Recruits the PR-DUB complex to specific gene-regulatory regions (PubMed:20805357). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

Alternative names

INO80S, YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1

Recommended products

Rabbit Recombinant Multiclonal YY1 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human YY1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Recombinant Fragment Protein within Human YY1. Database link P25490
Clone number
RP23040257
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

YY1 also known as Yin Yang 1 is a transcription factor that regulates gene expression by binding to specific DNA sequences. It is a protein with a molecular mass of approximately 68 kDa and is ubiquitously expressed in many tissues including liver lung and kidney. Functionally YY1 can both activate and repress transcription making it versatile in regulating gene activity. It interacts with several co-factors and other proteins influencing chromatin structure and gene expression.

Biological function summary

Yin Yang 1 plays an important role in various cellular processes such as cell proliferation differentiation and apoptosis. It is part of a chromatin remodeling complex where it assists in modifying the architecture of chromatin to control gene activity. Due to its ability to bind to diverse DNA motifs YY1 is involved in embryonic development and cellular differentiation. Additionally it interacts with other transcription factors to coordinate the expression of genes critical for cell cycle progression and survival.

Pathways

The Yin Yang 1 transcription factor is integrally involved in several key signaling pathways. It plays a notable role in the Wnt signaling pathway which is vital for controlling cell growth and differentiation. YY1 interacts with proteins such as β-catenin an important player in the Wnt pathway to mediate its transcriptional effects. Furthermore it also functions within the mTOR signaling pathway associating with proteins like mTOR itself to impact cell metabolism and growth.

Associated diseases and disorders

Alterations in the expression or function of YY1 have been implicated in several conditions. Dysregulation of YY1 has strong associations with cancer as its interactions with proteins like MYC can lead to abnormal cell proliferation and tumor development. Additionally its role in neuronal differentiation links it with neurological disorders such as Alzheimer's disease. In these contexts YY1's interaction with other transcription factors and regulatory proteins influences disease progression and severity.

Product promise

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420)

    YY1 Immunocytochemistry/ Immunofluorescence staining of DU 145 cells using rabbit Anti-YY1 antibody

    Immunofluorescent analysis of DU 145 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous YY1 with ab313420 at 1/100 dilution, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of YY1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of YY1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

  • Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420), expandable thumbnail

    Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420)

    YY1 Western blot staining using rabbit Anti-YY1 antibody

    All lanes: Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420) at 1/500 dilution

    Lane 1: Non-transfected DU145 whole cell lysate

    Lane 2: Non-specific scrambled siRNA transfected DU145 cell lysate

    Lane 3: YY1 knockdown DU145 whole cell lysate

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Observed band size: 55 kDa

  • Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420), expandable thumbnail

    Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420)

    YY1 Western blot staining using rabbit Anti-YY1 antibody

    All lanes: Western blot - Anti-YY1 Antibody [RP23040257] - Nuclear Loading Control (ab313420) at 1/500 dilution

    Lane 1: Modified nuclear extracts (1% SDS) from LNCap cells at 30 µg

    Lane 2: Modified nuclear extracts (1% SDS) from U-2 OS cells at 30 µg

    Lane 3: Modified nuclear extracts (1% SDS) from MCF-7 cells at 30 µg

    Lane 4: Modified nuclear extracts (1% SDS) from MDA-MB-231 cells at 30 µg

    Lane 5: Modified nuclear extracts (1% SDS) from HCT 166 cells at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Observed band size: 55 kDa

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Product protocols

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