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AB170925

Anti-ZAN antibody [EPR11209]

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(1 Publication)

Rabbit Recombinant Monoclonal ZAN antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Zonadhesin, ZAN

1 Images
Western blot - Anti-ZAN antibody [EPR11209] (AB170925)
  • WB

Supplier Data

Western blot - Anti-ZAN antibody [EPR11209] (AB170925)

All lanes:

Western blot - Anti-ZAN antibody [EPR11209] (ab170925) at 1/1000 dilution

All lanes:

Mouse testis lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 306 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11209

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZAN also known as Zonadhesin is a sperm-specific protein involved in the binding of sperm to the egg's zona pellucida. It has a molecular mass of approximately 280 kDa. Zonadhesin's expression is primarily detected in the testis particularly in spermatogenic cells. This protein plays an essential role during fertilization by mediating the initial adherence of sperm to the egg surface which is an important step in the fertilization process.
Biological function summary

This protein enables the gamete interaction necessary for successful reproduction. Zonadhesin functions by recognizing and binding to specific glycoproteins on the egg's zona pellucida. This interaction facilitates the penetration process allowing the sperm to deliver its genetic material into the egg. Zonadhesin operates alone and doesn’t join any known protein complex highlighting its autonomous action in gamete recognition.

Pathways

Zonadhesin's role in sperm-egg recognition directly implicates it in reproductive pathways particularly the acrosome reaction pathway which prepares the sperm for binding and penetrating the egg. It works in conjunction with acrosomal enzymes like acrosin which further facilitate the penetration of the zona pellucida. Zonadhesin's function in this pathway ensures that the sperm can navigate the zona pellucida barrier and engage in fertilization.

Abnormalities in Zonadhesin expression or function may lead to fertility issues such as sperm incapacitation or male infertility. Dysfunction in this protein can disrupt sperm-zona pellucida interaction making successful fertilization challenging. In cases of infertility related to Zonadhesin there may be interest in the protein sperm antigen 17 (SP17) as it also plays a part in reproductive processes and imbalances or dysfunctions in either protein can contribute to reproductive disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.
See full target information ZAN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 68: PubMed39494460

2024

Alarin regulates RyR2 and SERCA2 to improve cardiac function in heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Jinshuang Li,Dawei Xu,Ce Shi,Chunqi Cheng,Ziheng Xu,Xingjuan Gao,Yong Cheng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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