Anti-ZAP70 antibody [ZAP-03]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Intracellular Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations : T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Flow cytometry intracellular staining pattern of human peripheral whole blood using ab38871 (concentration in sample 9 μg/ml, GAM APC).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using ab38871 (concentration in sample 9 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 μl reagent / 100 μl of peripheral whole blood).

• WB

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Western blot - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel; Reducing conditions. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

Lane 1:

Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/mL

Lane 2:

Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/mL

Lane 3:

Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/mL

Lane 4:

Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/mL

Lane 5:

ZAP-70 specific antibody 1E7.2 at 1 µg/mL

Lane 6:

Isotypic control PPV-06 (mouse IgG1).

All lanes:

HPB-ALL peripheral blood leukemia T-cell line

Predicted band size: 69 kDa

Observed band size: 55 kDa,70 kDa

false

• WB

Unknown

Western blot - Anti-ZAP70 antibody [ZAP-03] (AB38871)

Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.

Lanes 1 - 2:

Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/mL

Lanes 3 - 4:

Isotypic control PPV-06 (mouse IgG1)

Lanes 1 and 3:

HPB-ALL cell line

Lanes 2 and 4:

RAMOS cell line

Predicted band size: 69 kDa

Observed band size: 70 kDa

false