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AB77085

Anti-ZBRK1 antibody

4

(5 Reviews)

|

(3 Publications)

Rabbit Polyclonal ZBRK1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZNF350.

View Alternative Names

ZBRK1, ZNF350, Zinc finger protein 350, KRAB zinc finger protein ZFQR, Zinc finger and BRCA1-interacting protein with a KRAB domain 1, Zinc finger protein ZBRK1

1 Images
Western blot - Anti-ZBRK1 antibody (AB77085)
  • WB

Unknown

Western blot - Anti-ZBRK1 antibody (AB77085)

All lanes:

Western blot - Anti-ZBRK1 antibody (ab77085) at 1 µg/mL

Lane 1:

A-20 lysate at 15 µg

Lane 2:

A-20 lysate at 15 µg with blocking peptide

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ZNF350. The exact immunogen used to generate this antibody is proprietary information.

Q9GZX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZBRK1 also known as ZNF350 is a zinc finger protein with a mass of approximately 61 kDa. It functions as a transcriptional repressor regulating gene expression by binding to specific DNA sequences. This protein is expressed in various tissues with notable levels observed in the brain lungs and reproductive organs. ZBRK1 interacts with other proteins to modulate its transcriptional activity playing an important role in the precise control of genetic information.
Biological function summary

The ZBRK1 protein plays a significant role in DNA damage response and cell cycle regulation. It often forms a complex with BRCA1 aiding in the repair of damaged DNA and maintaining genomic stability. This interaction suggests an involvement in regulating cell proliferation and preventing the accumulation of genetic mutations that could otherwise lead to oncogenic transformation. ZBRK1's ability to repress transcription of certain genes ensures controlled cell growth and division.

Pathways

ZBRK1 is integrally involved in the DNA damage response pathway and the p53 signaling pathway. In the DNA damage response pathway ZBRK1 works alongside BRCA1 to repair double-strand breaks through homologous recombination a critical process for maintaining genetic fidelity. Within the p53 signaling pathway ZBRK1 influences the expression levels of genes involved in cell cycle arrest and apoptosis relating it to proteins such as p21 which mediates cell cycle inhibition.

ZBRK1 holds connections to breast cancer and ovarian cancer. Mutations or dysregulation in ZBRK1 can affect its interaction with BRCA1 impairing DNA repair mechanisms and predisposing cells to oncogenic transformations. The defective repair of DNA can lead to genetic instability a hallmark of cancer progression. Moreover abnormalities in the pathways involving ZBRK1 are associated with changes in cell cycle control and apoptotic processes aligning with the development of malignant tumors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.
See full target information ZNF350
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:6959-76 PubMed25749518

2015

ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer.

Applications

IF

Species

Unspecified reactive species

Ke Chen,Gan Yu,Kiranmai Gumireddy,Anping Li,Weimin Yao,Lu Gao,Shuliang Chen,Jun Hao,Ji Wang,Qihong Huang,Hua Xu,Zhangqun Ye

Nucleic acids research 42:7012-27 PubMed24829459

2014

Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1.

Applications

ChIP

Species

Unspecified reactive species

Mario R Calderon,Mark Verway,Radia Ouelaa Benslama,Mirela Birlea,Manuella Bouttier,Vassil Dimitrov,Sylvie Mader,John H White

Human molecular genetics 20:104-14 PubMed20926453

2010

The KRAB-containing zinc-finger transcriptional regulator ZBRK1 activates SCA2 gene transcription through direct interaction with its gene product, ataxin-2.

Applications

WB, ChIP

Species

Human, Human

Linda Hallen,Holger Klein,Carola Stoschek,Silke Wehrmeyer,Ute Nonhoff,Markus Ralser,Jeannine Wilde,Christina Röhr,Michal R Schweiger,Kurt Zatloukal,Martin Vingron,Hans Lehrach,Zoltán Konthur,Sylvia Krobitsch
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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