AB117786

Anti-ZBT10 antibody

Name: Anti-ZBT10 antibody (ab117786) | Abcam
Brand: Abcam Limited
SKU: AB117786

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal ZBT10 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZBTB10 aa 50-100.

View Alternative Names

RINZF, RINZFC, ZBTB10, Zinc finger and BTB domain-containing protein 10, Zinc finger protein RIN ZF

2 Images

Unknown

Immunoprecipitation - Anti-ZBT10 antibody (AB117786)

Detection of ZBT10 by Western Blot of Immunprecipitate.
ab117786 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117786 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-ZBT10 antibody (ab117786)

Predicted band size: 95 kDa

false

Unknown

Western blot - Anti-ZBT10 antibody (AB117786)

All lanes:

Western blot - Anti-ZBT10 antibody (ab117786) at 0.1 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

Jurkat whole cell lysates at 50 µg

Predicted band size: 95 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZBTB10 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q96DT7

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab117786 was affinity purified using an epitope specific to ZBT10 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ZBT10 also known as Zinc Finger and BTB Domain Containing 10 acts as a transcription factor. Its molecular mass is approximately 55 kDa. This protein is expressed mainly in liver cells and several immune-related tissues showing a diverse expression pattern. ZBT10 contains a BTB/POZ domain which interacts with nuclear chromatin to regulate gene transcription. This protein contains specific zinc finger motifs that play a role in binding to DNA sequences.

Biological function summary

Zinc Finger and BTB Domain Containing 10 is involved in regulating cell differentiation and proliferation. It plays a critical role in hematopoiesis processes and may take part in muscle tissue development. ZBT10 often forms a complex with other transcription factors aiding precise control of gene expression related to cell cycle progression. By modulating different transcriptional activities ZBT10 influences the expression of genes involved in developmental pathways.

Pathways

Zinc Finger and BTB Domain Containing 10 participates in the Notch signaling pathway which determines cell fate decisions. This protein interacts with CSL (CBF1/Su(H)/LAG-1) important in transducing activated Notch signals. ZBT10 indirectly affects the WNT signaling pathway by regulating transcription factors involved in cell proliferation and migration. In these pathways ZBT10 holds a position alongside key proteins like beta-catenin and jagged proteins.

Aberrations in ZBT10 expression correlate with certain cancers particularly involving the liver. Elevated or reduced levels of this protein may influence oncogenesis by altering cell proliferation rates. Additionally ZBT10 has been linked to immunological disorders by impacting immune cell differentiation; it connects with proteins such as STAT3 a critical player in immune response regulation. Understanding ZBT10's role provides insights into potential therapeutic targets for these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May be involved in transcriptional regulation.

See full target information ZBTB10

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37627542

2023

Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Fosso,Manuela Leo,Livio Muccillo,Vittorio Maria Mandrone,Maria Chiara Di Meo,Annamaria Molinario,Ettore Varricchio,Lina Sabatino

Cell death & disease 13:252 PubMed35306527

2022

Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ching Wen,Wei-Yu Chen,Van Thi Ngoc Tram,Hsiu-Lien Yeh,Wei-Hao Chen,Kuo-Ching Jiang,Wassim Abou-Kheir,Jiaoti Huang,Michael Hsiao,Yen-Nien Liu

Developmental cell 40:523-536.e6 PubMed28350986

2017

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Nakajima,Kimiko Yamamoto,Sobhika Agarwala,Kenta Terai,Hajime Fukui,Shigetomo Fukuhara,Koji Ando,Takahiro Miyazaki,Yasuhiro Yokota,Etienne Schmelzer,Heinz-Georg Belting,Markus Affolter,Virginie Lecaudey,Naoki Mochizuki

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZBT10 antibody

Immunoprecipitation - Anti-ZBT10 antibody (AB117786)

Western blot - Anti-ZBT10 antibody (AB117786)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise