AB117756

Anti-ZBTB2 antibody

Name: Anti-ZBTB2 antibody (ab117756) | Abcam
Brand: Abcam Limited
SKU: AB117756
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ZBTB2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZBTB2 aa 450 to C-terminus.

View Alternative Names

KIAA1483, ZNF437, ZBTB2, Zinc finger and BTB domain-containing protein 2

2 Images

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Immunoprecipitation - Anti-ZBTB2 antibody (AB117756)

Detection of ZBTB2 by Western Blot of Immunprecipitate.
ab117756 at 1µg/ml staining ZBTB2 in 293T whole cell lysate immunoprecipitated using ab117756 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZBTB2 antibody (ab117756)

Predicted band size: 57 kDa

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Western blot - Anti-ZBTB2 antibody (AB117756)

All lanes:

Western blot - Anti-ZBTB2 antibody (ab117756) at 0.04 µg/mL

Lane 1:

293T cell line lysate at 50 µg

Lane 2:

293T cell line lysate at 15 µg

Lane 3:

Jurkat cell line lysate at 50 µg

Lane 4:

HeLa cell line lysate at 50 µg

Lane 5:

NIH3T3 cell line lysate at 50 µg

Predicted band size: 57 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZBTB2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N680

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab117756 was affinity purified using an epitope specific to ZBTB2 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZBTB2 also known as Zinc finger and BTB domain-containing protein 2 is a transcriptional regulator with a molecular mass of approximately 95 kDa. It operates by binding DNA through its zinc finger motifs and recruiting chromatin remodeling complexes via the BTB/POZ domain affecting gene expression. You can find this protein expressed in various tissues with higher presence notably in the testis brain and some tumor tissues. As a result its expression pattern suggests a significant role in cellular proliferation and differentiation.

Biological function summary

ZBTB2 influences gene transcription and modulates cell cycle progression. It does not work alone but often forms part of transcriptional complexes. Its role in repressing or activating transcription depends on its interaction partners impacting key cellular processes. The protein’s ability to regulate numerous downstream effectors underlines its importance in maintaining cellular homeostasis and normal cellular function.

Pathways

Studies show ZBTB2 involvement in the p53 signaling and Wnt pathways which are important for regulating cell cycle and apoptosis. ZBTB2 interacts with E2F1 a regulator of cell cycle progression and negatively influences p53 activity reducing its transcriptional response. This interaction links ZBTB2 to cell growth and survival pathways making it a focus for understanding complex regulatory networks within cells.

Researchers find ZBTB2 correlated with cancer and neurodevelopmental disorders. Aberrant expression of ZBTB2 has been observed in various malignancies such as colorectal and gastric cancers where it modulates oncogenic pathways. It shows interaction with the MYC proto-oncogene protein enhancing transcriptional programs associated with cell proliferation and tumor development. In neurodevelopmental contexts altered ZBTB2 function can affect neural gene expression contributing to disorders like autism spectrum disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in transcriptional regulation.

See full target information ZBTB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biological trace element research : PubMed41034598

2025

Minimal Risk Doses of Cadmium Exposure Induce Histological and Functional Alterations in the Brown Adipose Tissue of Wistar Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Enrique Sarmiento-Ortega,David Castillo-Pérez,Diana Moroni-González,Alfonso Diaz,Rubén Vázquez-Roque,Eduardo Brambila,Samuel Treviño

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZBTB2 antibody

Immunoprecipitation - Anti-ZBTB2 antibody (AB117756)

Western blot - Anti-ZBTB2 antibody (AB117756)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise