Rat Recombinant Monoclonal ZBTB20 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
IgG2a
Rat
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | |
---|---|
Mouse | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.077 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.077 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses (By similarity). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).
Zfp288, Zfp288, Zbtb20, Zinc finger and BTB domain-containing protein 20, BTB/POZ domain zinc finger factor HOF, Zinc finger protein 288
Rat Recombinant Monoclonal ZBTB20 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
IgG2a
Rat
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
4A3
Ion exchange chromatography
Blue Ice
+4°C
+4°C
Do Not Freeze
ab252244 is the carrier-free version of Anti-ZBTB20 antibody [4A3] ab243143.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
ZBTB20 also known as ZFP288 or HOF functions as a transcription factor in the body. This protein has a molecular mass of approximately 79 kDa. It is expressed in several tissues with high levels found in the brain liver and pancreas. The protein contains zinc finger domains which assist in binding to specific DNA sequences regulating gene expression. It plays a role in the development and cellular differentiation by modulating target gene networks.
ZBTB20 influences various processes integral to cell growth and development. It participates in pathways associated with the regulation of gene expression and acts as a repressor for some genes. ZBTB20 does not primarily form part of a complex but interacts with other transcriptional regulators. The protein modulates hepatocyte proliferation and maintains neuronal function by affecting gene transcription in these regions.
Several biological processes depend on effective interaction with ZBTB20. This protein is involved in the insulin signaling pathway which affects glucose metabolism and homeostasis. ZBTB20 interacts with other significant proteins such as PDX1 to maintain pancreatic functions. Additionally it plays a minor role in the JAK-STAT pathway which influences immune response and growth through interaction with STAT proteins.
ZBTB20 exhibits connections to several health conditions specifically metabolic and neurological disorders. For instance mutations or dysregulation of ZBTB20 correlate with diabetes due to its involvement in insulin regulation. Additionally abnormalities in ZBTB20 expression link to intellectual disabilities influencing brain function. The protein interacts with SOX2 a critical regulator of neuronal gene expression contributing to these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 cerebrum tissue labeling ZBTB20 with Anti-ZBTB20 antibody [4A3] ab243143 at 0.077μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse E14.5 cerebrum(PMID: 20308569). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZBTB20 antibody [4A3] ab243143).
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ZBTB20 with Anti-ZBTB20 antibody [4A3] ab243143 at 0.077μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse pancreas (PMID: 22374165). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZBTB20 antibody [4A3] ab243143).
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling ZBTB20 with Anti-ZBTB20 antibody [4A3] ab243143 at 0.077μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse hippocampus (PMID: 11744704). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZBTB20 antibody [4A3] ab243143).
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling ZBTB20 with Anti-ZBTB20 antibody [4A3] ab243143 at 0.077μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on rat hippocampus (PMID: 11744704). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZBTB20 antibody [4A3] ab243143).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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