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Rat Recombinant Monoclonal ZBTB20 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (AB252244), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (AB252244), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (AB252244), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (AB252244), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

0.077 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

0.077 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses (By similarity). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).

Alternative names

Recommended products

Rat Recombinant Monoclonal ZBTB20 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

4A3

Purification technique

Ion exchange chromatography

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab252244 is the carrier-free version of Anti-ZBTB20 antibody [4A3] ab243143.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZBTB20 also known as ZFP288 or HOF functions as a transcription factor in the body. This protein has a molecular mass of approximately 79 kDa. It is expressed in several tissues with high levels found in the brain liver and pancreas. The protein contains zinc finger domains which assist in binding to specific DNA sequences regulating gene expression. It plays a role in the development and cellular differentiation by modulating target gene networks.

Biological function summary

ZBTB20 influences various processes integral to cell growth and development. It participates in pathways associated with the regulation of gene expression and acts as a repressor for some genes. ZBTB20 does not primarily form part of a complex but interacts with other transcriptional regulators. The protein modulates hepatocyte proliferation and maintains neuronal function by affecting gene transcription in these regions.

Pathways

Several biological processes depend on effective interaction with ZBTB20. This protein is involved in the insulin signaling pathway which affects glucose metabolism and homeostasis. ZBTB20 interacts with other significant proteins such as PDX1 to maintain pancreatic functions. Additionally it plays a minor role in the JAK-STAT pathway which influences immune response and growth through interaction with STAT proteins.

Associated diseases and disorders

ZBTB20 exhibits connections to several health conditions specifically metabolic and neurological disorders. For instance mutations or dysregulation of ZBTB20 correlate with diabetes due to its involvement in insulin regulation. Additionally abnormalities in ZBTB20 expression link to intellectual disabilities influencing brain function. The protein interacts with SOX2 a critical regulator of neuronal gene expression contributing to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com