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AB106554

Anti-ZBTB4 antibody

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(3 Publications)

Rabbit Polyclonal ZBTB4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZBTB4.

View Alternative Names

KIAA1538, ZBTB4, Zinc finger and BTB domain-containing protein 4, KAISO-like zinc finger protein 1, KAISO-L1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZBTB4 antibody (AB106554)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZBTB4 antibody (AB106554)

Immunofluorescence of ZBTB4 in Human brain tissue with ab106554 at 20 ug/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB4 antibody (AB106554)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB4 antibody (AB106554)

Immunohistochemistry of ZBTB4 in human brain tissue with ab106554 at 2.5 µg/ml.

Western blot - Anti-ZBTB4 antibody (AB106554)
  • WB

Unknown

Western blot - Anti-ZBTB4 antibody (AB106554)

All lanes:

Western blot - Anti-ZBTB4 antibody (ab106554) at 1 µg/mL

All lanes:

SK-N-SH cell lysate at 15 µg

Predicted band size: 105 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human ZBTB4. The exact immunogen used to generate this antibody is proprietary information.

Q9P1Z0

Specificity

At least four isoforms of ZBTB4 are known to exist; ab106554 will only recognize the longest isoform. This antibody is predicted not to cross-react with other ZBTB protein family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZBTB4 also known as Zinc Finger and BTB Domain-Containing Protein 4 is a transcriptional repressor with a mass of approximately 140 kDa. ZBTB4 contains a BTB/POZ domain and several zinc finger motifs allowing it to bind DNA and suppress gene expression. It is expressed in various tissues with higher levels observed in the brain lung and liver. The protein primarily localizes in the nucleus where it regulates the transcription of specific target genes.
Biological function summary

ZBTB4 interacts with other proteins as a part of transcriptional repression complexes. Its role includes modulating gene expression linked to cellular proliferation and differentiation. ZBTB4 often works together with corepressors such as CtBP which enhances its repressive function. Through these interactions it helps maintain genomic stability and manage cell cycle progression.

Pathways

Research indicates that ZBTB4 is an important component of the p53 signaling and apoptosis pathways. It plays a role in mediating the expression of genes involved in cell cycle arrest and apoptosis which are critical in response to DNA damage. The p53 tumor suppressor protein a well-known pathway partner interacts with ZBTB4 to control cell fate decisions under stress conditions.

ZBTB4 is implicated in cancer progression and development. Alterations in ZBTB4 expression or function can contribute to tumorigenesis often relating to its connections with p53 and MDM2 proteins. This linkage points to a role in breast cancer and gastric cancer where deregulation of cell cycle and apoptosis pathways is common. Understanding the involvement of ZBTB4 in these pathways could offer insights into new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA (PubMed : 16354688). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (By similarity).
See full target information ZBTB4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:114-128 PubMed38151999

2023

Demethylase FTO inhibits the occurrence and development of triple-negative breast cancer by blocking m A-dependent miR-17-5p maturation-induced ZBTB4 depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Ni,Xiaoyun Lu,Xiangxiang Gao,Conghui Jin,Junfeng Mao

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 25:706-720 PubMed36538280

2022

miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Qing Zhang,Beihua Kong

Experimental and therapeutic medicine 16:4537-4545 PubMed30542402

2018

MicroRNA-106b promotes the proliferation, migration and invasion of retinoblastoma cells by inhibiting the expression of ZBTB4 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Bu,Yanhui Wang,Xiangrong Min
View all publications
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Product promise

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