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AB86330

Anti-ZBTB40 antibody

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(1 Publication)

Rabbit Polyclonal ZBTB40 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZBTB40 aa 1100-1200.

View Alternative Names

KIAA0478, ZBTB40, Zinc finger and BTB domain-containing protein 40

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (AB86330)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (AB86330)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-ZBTB40 antibody (AB86330)
  • IP

Unknown

Immunoprecipitation - Anti-ZBTB40 antibody (AB86330)

Detection of ZBTB40 by Western Blot of Immunprecipitate.
ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ZBTB40 antibody (ab86330)

Predicted band size: 138 kDa

false

Western blot - Anti-ZBTB40 antibody (AB86330)
  • WB

Unknown

Western blot - Anti-ZBTB40 antibody (AB86330)

All lanes:

Western blot - Anti-ZBTB40 antibody (ab86330) at 0.1 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

293T whole cell lysates at 5 µg

Predicted band size: 138 kDa

Observed band size: 170 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ZBTB40 aa 1100-1200. The exact immunogen used to generate this antibody is proprietary information.

Q9NUA8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZBTB40 also known as Zinc Finger and BTB Domain-Containing Protein 40 functions mainly as a transcription factor involved in gene regulation. This protein has a molecular weight of approximately 87 kDa. Researchers have observed its expression in various tissues though it is found at higher levels in skeletal tissues. The presence of zinc finger domains suggests its role in DNA binding influencing the transcription of specific genes.
Biological function summary

ZBTB40 participates in bone-related functions. It is not known to be part of any complex. Scientists have associated it with osteoblast differentiation and bone formation indicating its role in skeletal development. The regulation of genes by ZBTB40 influences cellular processes that contribute to the structural and functional integrity of bone tissue.

Pathways

ZBTB40's activities align with bone morphogenetic protein (BMP) signaling and Wnt signaling pathways. These pathways are critical for bone growth and remodeling. ZBTB40 interacts with proteins involved in these pathways such as BMPs and Wnt family proteins modulating their effects downstream. These interactions help coordinate the complex signaling necessary for maintaining bone health.

ZBTB40 has links to osteoporosis and skeletal dysplasia. Researchers have studied its regulatory functions in bone mineral density connecting it to conditions of bone fragility and poor bone development. The protein also interacts with RUNX2 an important factor involved in skeletal system health exacerbating or mitigating disease progression based on ZBTB40's activity levels. Understanding these connections provides insights into potential therapeutic targets for these bone-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZBTB40
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 11:22 PubMed31918758

2020

IGF-1 enhances BMSC viability, migration, and anti-apoptosis in myocardial infarction via secreted frizzled-related protein 2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhuo Lin,Xinyue Liu,Haoxiao Zheng,Xiaohui Huang,Yu Wu,Anqing Huang,Hailan Zhu,Yunzhao Hu,Weiyi Mai,Yuli Huang
View all publications
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Product promise

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