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AB222830

Anti-ZBTB46 antibody

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Rabbit Polyclonal ZBTB46 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB46 aa 150-400.

View Alternative Names

BTBD4, ZNF340, ZBTB46, Zinc finger and BTB domain-containing protein 46, BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, Zinc finger protein 340, BZEL

3 Images
Western blot - Anti-ZBTB46 antibody (AB222830)
  • WB

Supplier Data

Western blot - Anti-ZBTB46 antibody (AB222830)

All lanes:

Western blot - Anti-ZBTB46 antibody (ab222830) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 5:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (AB222830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (AB222830)

Paraffin-embedded human rectum tissue stained for ZBTB46 using ab222830 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (AB222830)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody (AB222830)

Paraffin-embedded human brain tissue stained for ZBTB46 using ab222830 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZBTB46 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q86UZ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZBTB46 also known as zbtb46 Zfp405 or BTBD4 is a transcription factor with a molecular weight of approximately 60 kDa. It belongs to the BTB-ZF protein family and mainly expresses in dendritic cells and endothelial cells. This protein plays an important role in regulating gene expression by binding to DNA sequences through its zinc-finger domains. ZBTB46 serves as a specific marker for conventional dendritic cells which distinguishes them from other immune cells.
Biological function summary

The functioning of ZBTB46 involves the regulation of immune system responses and cellular development processes. ZBTB46 does not form a larger protein complex. Its activity impacts the maturation and function of certain immune cells especially those involved in presenting antigens to T cells. Through this regulation ZBTB46 influences various immune processes including inflammation and the maintenance of immune homeostasis helping to prevent excessive immune responses.

Pathways

ZBTB46 plays a role in the innate immune response and hematopoiesis. In the innate immune response it helps dictate the development of dendritic cells interacting with key proteins such as ID2 and E2-2. During hematopoiesis it guides the differentiation of multipotent progenitors toward specific cell lineages maintaining balanced production of immune cells. ZBTB46’s involvement in these pathways confirms its importance in both immune system and blood cell development.

ZBTB46 has associations with immunological disorders such as autoimmune diseases and inflammatory conditions. Dysregulation of ZBTB46 expression can contribute to abnormal immune responses potentially leading to chronic inflammation or autoimmunity. In conditions like systemic lupus erythematosus ZBTB46 dysregulation may occur in connection with proteins like STAT3 influencing disease progression and severity. This establishes ZBTB46 as a potential target for therapeutic interventions aiming to modulate immune function in such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a transcriptional repressor for PRDM1.
See full target information ZBTB46
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Product promise

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