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Mouse Monoclonal ZBTB46 antibody. Suitable for Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZBTB46.

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Images

Flow Cytometry - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (AB277100), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (AB277100), expandable thumbnail
  • Protein Array - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (AB277100), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ZBTB46. Database link Q86UZ6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytProtein ArrayIHC-P
Human
Tested
Expected
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

Functions as a transcriptional repressor for PRDM1.

Alternative names

Zinc finger and BTB domain-containing protein 46, BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, Zinc finger protein 340, BZEL, ZNF340, BTBD4, ZBTB46

Recommended products

Mouse Monoclonal ZBTB46 antibody. Suitable for Flow Cyt, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZBTB46.

Alternative names

Zinc finger and BTB domain-containing protein 46, BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, Zinc finger protein 340, BZEL, ZNF340, BTBD4, ZBTB46

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human ZBTB46. Database link Q86UZ6
Clone number

PCRP-ZBTB46-2B8

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from bioreactor concentrate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZBTB46 also known as zbtb46 Zfp405 or BTBD4 is a transcription factor with a molecular weight of approximately 60 kDa. It belongs to the BTB-ZF protein family and mainly expresses in dendritic cells and endothelial cells. This protein plays an important role in regulating gene expression by binding to DNA sequences through its zinc-finger domains. ZBTB46 serves as a specific marker for conventional dendritic cells which distinguishes them from other immune cells.

Biological function summary

The functioning of ZBTB46 involves the regulation of immune system responses and cellular development processes. ZBTB46 does not form a larger protein complex. Its activity impacts the maturation and function of certain immune cells especially those involved in presenting antigens to T cells. Through this regulation ZBTB46 influences various immune processes including inflammation and the maintenance of immune homeostasis helping to prevent excessive immune responses.

Pathways

ZBTB46 plays a role in the innate immune response and hematopoiesis. In the innate immune response it helps dictate the development of dendritic cells interacting with key proteins such as ID2 and E2-2. During hematopoiesis it guides the differentiation of multipotent progenitors toward specific cell lineages maintaining balanced production of immune cells. ZBTB46’s involvement in these pathways confirms its importance in both immune system and blood cell development.

Associated diseases and disorders

ZBTB46 has associations with immunological disorders such as autoimmune diseases and inflammatory conditions. Dysregulation of ZBTB46 expression can contribute to abnormal immune responses potentially leading to chronic inflammation or autoimmunity. In conditions like systemic lupus erythematosus ZBTB46 dysregulation may occur in connection with proteins like STAT3 influencing disease progression and severity. This establishes ZBTB46 as a potential target for therapeutic interventions aiming to modulate immune function in such disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100), expandable thumbnail

    Flow Cytometry - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100)

    Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ZBTB46 using ab277100 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for ZBTB46 using ab277100 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100), expandable thumbnail

    Protein Array - Anti-ZBTB46 antibody [PCRP-ZBTB46-2B8] (ab277100)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277100.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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