Rabbit Polyclonal ZBTB48 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB48 aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZBTB48 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P10074
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Target data
Function
Telomere-binding protein that acts as a regulator of telomere length (PubMed:28082411, PubMed:28500257). Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat (PubMed:28082411, PubMed:28500257). Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres (PubMed:28082411). Also acts as a transcription regulator that binds to promoter regions (PubMed:24382891, PubMed:28500257, PubMed:7969177). Regulates expression of a small subset of genes, including MTFP1 (PubMed:28500257). Regulates expression the J and/or S elements in MHC II promoter (PubMed:7969177). Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A (PubMed:24382891).
Alternative names
HKR3, TZAP, ZNF855, ZBTB48, Telomere zinc finger-associated protein, Krueppel-related zinc finger protein 3, Zinc finger and BTB domain-containing protein 48, Zinc finger protein 855, hKR3
Recommended products
Rabbit Polyclonal ZBTB48 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB48 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZBTB48 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P10074
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZBTB48 also known as Telomeric Zinc Finger Associated Protein (TZAP) is a protein with a mass of approximately 56 kDa. It binds to telomeric repeat-binding factor 1 (TRF1) and limits telomere length. ZBTB48 contains zinc finger domains that allow it to interact with specific DNA sequences. It is predominantly expressed in the nucleus of human cells where it influences telomere dynamics.
- Biological function summary
ZBTB48 serves as a critical component in regulating telomere length and maintaining genomic stability. As part of the shelterin complex it assists in the protection of chromosome ends. ZBTB48 helps prevent telomere fusion and activates pathways that induce the shortening of excessively long telomeres. This regulation is essential for preventing dysregulation in cell division processes.
- Pathways
ZBTB48 plays an important role in telomere maintenance and cellular aging pathways. It interacts with proteins like TRF1 and TRF2 to help manage telomere integrity. In the telomere shortening pathway ZBTB48 identifies and binds to lengthy telomeres promoting their reduction. This activity connects it with the telomerase pathway although it acts in opposition to telomerase by ensuring telomeres do not become too long.
- Associated diseases and disorders
Dysregulation of ZBTB48 activity connects to cancer and aging-related diseases. Abnormal function can lead to inappropriate telomere elongation contributing to tumorigenesis. The protein's interactions with TRF1 and TRF2 are significant in these contexts as disruptions in these interactions can lead to chromosomal instability. Understanding ZBTB48's role may offer insights into therapeutic approaches for cancer treatment and aging disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-ZBTB48 antibody - N-terminal (ab225935)
All lanes: Western blot - Anti-ZBTB48 antibody - N-terminal (ab225935) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4: U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5: Mouse liver tissue lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB48 antibody - N-terminal (ab225935)
Paraffin-embedded human spleen tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB48 antibody - N-terminal (ab225935)
Paraffin-embedded human lung tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
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