AB70802

Anti-ZC3H13 antibody

Name: Anti-ZC3H13 antibody (ab70802) | Abcam
Brand: Abcam Limited
SKU: AB70802
Price: 545 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(7 Publications)

Rabbit Polyclonal ZC3H13 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ZC3H13.

View Alternative Names

KIAA0853, ZC3H13, Zinc finger CCCH domain-containing protein 13

2 Images

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Western blot - Anti-ZC3H13 antibody (AB70802)

All lanes:

Western blot - Anti-ZC3H13 antibody (ab70802) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 197 kDa

Observed band size: 200 kDa,240 kDa,85 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-ZC3H13 antibody (AB70802)

Detection of ZC3H13 by Western Blot of Immunoprecipitate.
ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZC3H13 antibody (ab70802)

Predicted band size: 197 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZC3H13. The exact immunogen used to generate this antibody is proprietary information.

Q5T200

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZC3H13 also known as zinc finger CCCH-type containing 13 is a protein with mass around 110 kDa. This protein is recognized for its role in post-transcriptional regulation of gene expression by mediating methylation of the N6 position of adenosine residues within RNA molecules. It possesses zinc finger motifs which allow it to bind to RNA and participate in RNA processing. ZC3H13 is expressed in various tissues including the liver lungs and brain indicating its widespread influence in physiological processes.

Biological function summary

ZC3H13 functions as part of the m6A methyltransferase complex where it interacts with other proteins like METTL3 and METTL14. This complex modifies mRNA influencing its stability localization and translation efficiency. Through these actions ZC3H13 plays an important role in regulating RNA metabolism impacting numerous biological activities such as cellular differentiation and response to environmental stress.

Pathways

The function of ZC3H13 integrates into both the mRNA processing and m6A methylation pathways. The protein maintains a strong link with RNA metabolism underpinning cellular processes critical for gene expression. In the pathways it associates with proteins like WTAP and KIAA1429 which coordinate to modulate mRNA methylation levels and stability therefore influencing gene expression patterns and cellular function.

Abnormal function or expression of ZC3H13 is associated with cancer and developmental disorders such as acute myeloid leukemia. ZC3H13's activity is often altered in these conditions contributing to dysregulation of gene expression and uncontrolled cell growth. In cancer its connections with proteins like METTL3 play a role in oncogenic transformation highlighting its potential as a therapeutic target for intervention strategies aiming to modulate mRNA methylation and stabilize normal cellular functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3'-UTR (By similarity). Controls embryonic stem cells (ESCs) pluripotency via its role in m6A methylation (By similarity). In the WMM complex, anchors component of the MACOM subcomplex in the nucleus (By similarity). Also required for bridging WTAP to the RNA-binding component RBM15 (RBM15 or RBM15B) (By similarity).

See full target information ZC3H13

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cartilage :19476035251339410 PubMed40433805

2025

ZC3H13 Promotes NSUN4-Mediated Chondrocyte Mitochondrial Dysfunction and Pyroptosis in Temporomandibular Joint Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Haotian Ren

Frontiers in immunology 13:976107 PubMed36091006

2022

Comprehensive characterization of tumor microenvironment and m6A RNA methylation regulators and its effects on PD-L1 and immune infiltrates in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Ji,Jian-An Zhang,Hejing Liu,Kehan Li,Zhi-Wei Wang,Xueqiong Zhu

Journal of oncology 2021:1328444 PubMed35003256

2021

ZC3H13 Inhibits the Progression of Hepatocellular Carcinoma through mA-PKM2-Mediated Glycolysis and Enhances Chemosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Qibo Wang,Haichuan Xie,Hao Peng,Jianjian Yan,Limin Han,Gang Ye

The Journal of biological chemistry 297:101248 PubMed34582888

2021

Wilms' tumor 1-associating protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Horiuchi,Takeshi Kawamura,Takao Hamakubo

Frontiers in endocrinology 10:415 PubMed31316467

2019

Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Boxian Huang,Chenyue Ding,Qinyan Zou,Wei Wang,Hong Li

Genes & development 32:415-429 PubMed29535189

2018

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the mA machinery component Wtap/Fl(2)d.

Applications

Unspecified application

Species

Unspecified reactive species

Philip Knuckles,Tina Lence,Irmgard U Haussmann,Dominik Jacob,Nastasja Kreim,Sarah H Carl,Irene Masiello,Tina Hares,Rodrigo Villaseñor,Daniel Hess,Miguel A Andrade-Navarro,Marco Biggiogera,Mark Helm,Matthias Soller,Marc Bühler,Jean-Yves Roignant

The Journal of biological chemistry 288:33292-302 PubMed24100041

2013

Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.

Applications

ICC/IF, WB

Species

Human, Human

Keiko Horiuchi,Takeshi Kawamura,Hiroko Iwanari,Riuko Ohashi,Makoto Naito,Tatsuhiko Kodama,Takao Hamakubo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZC3H13 antibody

Western blot - Anti-ZC3H13 antibody (AB70802)

Immunoprecipitation - Anti-ZC3H13 antibody (AB70802)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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