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AB169061

Anti-ZC3H14 antibody

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(2 Publications)

Rabbit Polyclonal ZC3H14 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZC3H14 aa 450-500.

View Alternative Names

Zinc finger CCCH domain-containing protein 14, Mammalian suppressor of tau pathology-2, Renal carcinoma antigen NY-REN-37, MSUT-2, ZC3H14

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Immunoprecipitation - Anti-ZC3H14 antibody (AB169061)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZC3H14 antibody (AB169061)

Detection of ZC3H14 in Immunoprecipitates of HEK293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab169061 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZC3H14 antibody (ab169061)

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human ZC3H14 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q6PJT7

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger CCCH domain-containing protein ZC3H14 also known as N4BP3 or DFP1 functions as a RNA-binding protein and weighs approximately 95 kDa. This protein is expressed in several tissues with higher levels observed in the brain. It performs a critical role in post-transcriptional regulation by binding to polyadenylated RNA. ZC3H14 might directly influence mRNA stability and decay which are key components of gene expression regulation.
Biological function summary

Beyond its RNA-binding capacity ZC3H14 makes a significant impact on neuronal function as it associates with particular RNA processing activities. Though not formally part of a complex it has interactions that suggest it collaborates with other proteins in the cell to perform its functions. Its activity is particularly important in maintaining proper levels of gene expression in neurons which is critical for their health and function.

Pathways

The work of ZC3H14 in post-transcriptional regulation affects important processes such as mRNA surveillance and RNA turnover pathways. These pathways play important roles in ensuring that aberrant mRNA transcripts do not accumulate in cells. Within these pathways ZC3H14 might engage with other proteins like UPF1 which is an important factor in nonsense-mediated decay. This interaction ensures only properly processed mRNAs are translated into proteins therefore maintaining cellular homeostasis.

Abnormal function or expression of ZC3H14 links to neurological conditions notably intellectual disabilities. The mutation or loss of function of this protein leads to improper mRNA processing and neuronal dysfunction. ZC3H14 also connects to disorders of protein homeostasis due to its involvement with RNA-binding proteins that regulate gene expression in the nervous system. Understanding its precise role and interactions may provide insights into therapeutic strategies for diseases such as these.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in poly(A) tail length control in neuronal cells. Binds the polyadenosine RNA oligonucleotides.
See full target information ZC3H14
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 296:100118 PubMed33234594

2020

An endogenous PI3K interactome promoting astrocyte-mediated neuroprotection identifies a novel association with RNA-binding protein ZC3H14.

Applications

Unspecified application

Species

Unspecified reactive species

Samih Alqawlaq,Izhar Livne-Bar,Declan Williams,Joseph D'Ercole,Sara W Leung,Darren Chan,Alessandra Tuccitto,Alessandro Datti,Jeffrey L Wrana,Anita H Corbett,Gerold Schmitt-Ulms,Jeremy M Sivak

Science advances 6:eaba5136 PubMed32596459

2020

Suppression of adenosine-to-inosine (A-to-I) RNA editome by death associated protein 3 (DAP3) promotes cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Han,Omer An,HuiQi Hong,Tim Hon Man Chan,Yangyang Song,Haoqing Shen,Sze Jing Tang,Jaymie Siqi Lin,Vanessa Hui En Ng,Daryl Jin Tai Tay,Fernando Bellido Molias,Priyankaa Pitcheshwar,Hui Qing Tan,Henry Yang,Leilei Chen
View all publications
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