AB232936

Anti-ZC3H14 antibody

Name: Anti-ZC3H14 antibody (ab232936) | Abcam
Brand: Abcam Limited
SKU: AB232936

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(1 Publication)

Rabbit Polyclonal ZC3H14 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZC3H14 aa 650 to C-terminus.

View Alternative Names

Zinc finger CCCH domain-containing protein 14, Mammalian suppressor of tau pathology-2, Renal carcinoma antigen NY-REN-37, MSUT-2, ZC3H14

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Supplier Data

Immunoprecipitation - Anti-ZC3H14 antibody (AB232936)

ZC3H14 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) with ab232936 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab232936 at 1 μg/ml.

Lane 1 : ab232936 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZC3H14 antibody (ab232936)

Predicted band size: 83 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human ZC3H14 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232936 was affinity purified using an epitope specific to ZC3H14 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger CCCH domain-containing protein ZC3H14 also known as N4BP3 or DFP1 functions as a RNA-binding protein and weighs approximately 95 kDa. This protein is expressed in several tissues with higher levels observed in the brain. It performs a critical role in post-transcriptional regulation by binding to polyadenylated RNA. ZC3H14 might directly influence mRNA stability and decay which are key components of gene expression regulation.

Biological function summary

Beyond its RNA-binding capacity ZC3H14 makes a significant impact on neuronal function as it associates with particular RNA processing activities. Though not formally part of a complex it has interactions that suggest it collaborates with other proteins in the cell to perform its functions. Its activity is particularly important in maintaining proper levels of gene expression in neurons which is critical for their health and function.

Pathways

The work of ZC3H14 in post-transcriptional regulation affects important processes such as mRNA surveillance and RNA turnover pathways. These pathways play important roles in ensuring that aberrant mRNA transcripts do not accumulate in cells. Within these pathways ZC3H14 might engage with other proteins like UPF1 which is an important factor in nonsense-mediated decay. This interaction ensures only properly processed mRNAs are translated into proteins therefore maintaining cellular homeostasis.

Abnormal function or expression of ZC3H14 links to neurological conditions notably intellectual disabilities. The mutation or loss of function of this protein leads to improper mRNA processing and neuronal dysfunction. ZC3H14 also connects to disorders of protein homeostasis due to its involvement with RNA-binding proteins that regulate gene expression in the nervous system. Understanding its precise role and interactions may provide insights into therapeutic strategies for diseases such as these.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

RNA-binding protein involved in the biogenesis of circular RNAs (circRNAs), which are produced by back-splicing circularization of pre-mRNAs (PubMed : 39461343). Acts by binding to both exon-intron boundary and 3'-UTR of pre-mRNAs to promote circRNA biogenesis through dimerization and the association with the spliceosome (PubMed : 39461343). Required for spermatogenesis via involvement in circRNA biogenesis (PubMed : 39461343). Regulates the pre-mRNA processing of ATP5MC1; preventing its degradation (PubMed : 27563065). Also binds the poly(A) tail of mRNAs; controlling poly(A) length in neuronal cells (PubMed : 17630287, PubMed : 24671764).

See full target information ZC3H14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 59:869-881.e6 PubMed38359832

2024

Multimodal mass spectrometry imaging identifies cell-type-specific metabolic and lipidomic variation in the mammalian liver.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Tian,Presha Rajbhandari,Jay Tarolli,Aubrianna M Decker,Taruna V Neelakantan,Tina Angerer,Fereshteh Zandkarimi,Helen Remotti,Gilles Frache,Nicholas Winograd,Brent R Stockwell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZC3H14 antibody

Immunoprecipitation - Anti-ZC3H14 antibody (AB232936)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise