Anti-ZC4H2 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC4H2 antibody (AB100924)

ab100924, at a 1/100 dilution, staining ZC4H2 in formalin fixed, paraffin embedded Human fetal colon tissue by Immunohistochemistry.

• WB

Unknown

Western blot - Anti-ZC4H2 antibody (AB100924)

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Western blot - Anti-ZC4H2 antibody (ab100924) at 1/500 dilution

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HeLa cell lysate

Predicted band size: 26 kDa

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• WB

CiteAb

Western blot - Anti-ZC4H2 antibody (AB100924)

ZC4H2 western blot using anti-ZC4H2 antibody ab100924. Publication image and figure legend from Vangeel, L., Janssens, A., et al., 2020, Int J Mol Sci, PubMed 32443528.

ab100924 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab100924 please see the product overview.

MAPPIT and co-IP show an interaction of ZC4H2 and TRPV4. (A) Volcano plot of the whole protein library tested in an initial MAPPIT screen, with the p-value in the function of the MAPPIT signal. Interesting ‘hits’ are located at the upper right corner. (B) Luciferase read-out of the top 17 hits identified in the MAPPIT screening. Baits cloned in the pSEL and pCLG vector background were tested in parallel and contained either the human N-terminus TRPV4 or an irrelevant protein (E. coli dihydrofolate reductase (eDHFR) or myelin and lymphocyte protein (MAL)). Additionally, an empty prey plasmid was used as a negative control. Positive assay controls are RRAD and GEM Like GTPase 2 (REM2) and EF-hand domain family member A1 (EFHA1), two proteins known to bind the cytokine receptor complex bait independently. (C) Human embryonic kidney (HEK)-293T cells were co-transfected with TRPV4-GFP and ZC4H2-mCherry (+) or TRPV4-GFP and mCherry (−). SDS-PAGE was performed for three conditions : the whole cell lysate, the fraction bound to GFP-Trap® beads, and the unbound (wash) fraction. Staining was done using specific antibodies for human TRPV4 (98 kDa), ZC4H2 (26 kDa), and β-actin (42 kDa). Note that in these experiments, TRPV4 and ZC4H2 are coupled to green fluorescent protein (GFP) and mCherry, respectively, which increases the molecular weights of the detected proteins by 27 kDa. In two independent experiments, we confirmed that there was negligible binding of mCherry and ZCH2-mCherry to the GFP-Trap® beads.

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