Rabbit Polyclonal ZC4H2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZC4H2 aa 1 to C-terminus.
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Publications
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- International journal of molecular sciences 21:2020The Zinc-Finger Domain Containing Protein ZC4H2 Interacts with TRPV4, Enhancing Channel Activity and Turnover at the Plasma Membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Vangeel et. al.PubMed 32443528
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZC4H2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQZ6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Plays a role in interneurons differentiation (PubMed:26056227). Involved in neuronal development and in neuromuscular junction formation.
Alternative names
HCA127, KIAA1166, ZC4H2, Zinc finger C4H2 domain-containing protein, Hepatocellular carcinoma-associated antigen 127
Recommended products
Rabbit Polyclonal ZC4H2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZC4H2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZC4H2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQZ6
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZC4H2 also known as Zinc Finger C4H2-Type (Androgen Receptor-Globin Transcription Factor Family Member 2) is a zinc finger protein with a molecular weight of approximately 24 kDa. This protein contains a C4H2-type zinc finger domain which suggests its role in DNA binding. ZC4H2 is expressed widely but it has higher expression levels observed in neural tissues. This broad expression pattern points towards its involvement in various cellular processes especially in the nervous system.
- Biological function summary
ZC4H2 plays a role in neuronal development and functions as a transcriptional regulator. It is a component of a larger protein complex that modulates gene expression. By binding to specific DNA sequences ZC4H2 influences the transcription of genes necessary for proper neuronal differentiation and function. It participates in processes that affect cell growth division and the maintenance of neural connections making it an important player in neural tissue homeostasis.
- Pathways
ZC4H2 participates in the Wnt signaling pathway which is important for developmental processes and the regulation of gene expression. This pathway involves interaction with proteins such as beta-catenin. Moreover ZC4H2 links to the Notch signaling pathway influencing cell fate determination through its interaction with Notch receptors. These pathways are essential for neural development suggesting ZC4H2’s significant role in brain development and its functional integrity.
- Associated diseases and disorders
Mutations in ZC4H2 have a connection to a rare neurodevelopmental disorder called Wieacker-Wolff Syndrome. This disorder involves intellectual disability muscle weakness and motor skills deficits. ZC4H2 mutations may disrupt neural development and function leading to these clinical manifestations. ZC4H2 also has an association with Rett Syndrome through its interaction with proteins involved in synapse formation and maintenance contributing to the neurodevelopmental deficit seen in this condition.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ZC4H2 antibody (ab100924)
All lanes: Western blot - Anti-ZC4H2 antibody (ab100924) at 1/500 dilution
All lanes: HeLa cell lysate
Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC4H2 antibody (ab100924)
ab100924, at a 1/100 dilution, staining ZC4H2 in formalin fixed, paraffin embedded Human fetal colon tissue by Immunohistochemistry.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ZC4H2 antibody (ab100924)
ZC4H2 western blot using anti-ZC4H2 antibody ab100924. Publication image and figure legend from Vangeel, L., Janssens, A., et al., 2020, Int J Mol Sci, PubMed 32443528.
ab100924 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab100924 please see the product overview.
MAPPIT and co-IP show an interaction of ZC4H2 and TRPV4. (A) Volcano plot of the whole protein library tested in an initial MAPPIT screen, with the p-value in the function of the MAPPIT signal. Interesting ‘hits’ are located at the upper right corner. (B) Luciferase read-out of the top 17 hits identified in the MAPPIT screening. Baits cloned in the pSEL and pCLG vector background were tested in parallel and contained either the human N-terminus TRPV4 or an irrelevant protein (E. coli dihydrofolate reductase (eDHFR) or myelin and lymphocyte protein (MAL)). Additionally, an empty prey plasmid was used as a negative control. Positive assay controls are RRAD and GEM Like GTPase 2 (REM2) and EF-hand domain family member A1 (EFHA1), two proteins known to bind the cytokine receptor complex bait independently. (C) Human embryonic kidney (HEK)-293T cells were co-transfected with TRPV4-GFP and ZC4H2-mCherry (+) or TRPV4-GFP and mCherry (−). SDS-PAGE was performed for three conditions: the whole cell lysate, the fraction bound to GFP-Trap® beads, and the unbound (wash) fraction. Staining was done using specific antibodies for human TRPV4 (98 kDa), ZC4H2 (26 kDa), and β-actin (42 kDa). Note that in these experiments, TRPV4 and ZC4H2 are coupled to green fluorescent protein (GFP) and mCherry, respectively, which increases the molecular weights of the detected proteins by 27 kDa. In two independent experiments, we confirmed that there was negligible binding of mCherry and ZCH2-mCherry to the GFP-Trap® beads.
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