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Rabbit Recombinant Monoclonal ZCCHC4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-ZCCHC4 antibody [EPR20492] (AB211325), expandable thumbnail
  • Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (AB211325), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Primates
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

This antibody works best on a semi-dry nitrocellulose membrane transfer system.

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

rRNA N6-methyltransferase that specifically methylates the adenine in position 4220 of 28S rRNA (PubMed:30531910, PubMed:31328227, PubMed:31695039, PubMed:31799605). N6-methylation of adenine(4220) in 28S rRNA is required for translation (PubMed:30531910, PubMed:31799605).

Alternative names

rRNA N(6)-adenosine-methyltransferase ZCCHC4, Zinc finger CCHC domain-containing protein 4, ZCCHC4

Recommended products

Rabbit Recombinant Monoclonal ZCCHC4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20492
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ZCCHC4 also known as zinc finger CCHC-type containing 4 is a protein that functions as a methyltransferase specifically modifying mRNA by adding a methyl group at the N6 position of adenine. It has an estimated mass of approximately 66 kDa. Expression of ZCCHC4 is observed mainly in the nucleus of cells and it is found across various human tissues indicating its widespread functional importance.

Biological function summary

The methyltransferase activity of ZCCHC4 plays an important role in post-transcriptional regulation of mRNA influencing its stability and translation efficiency. ZCCHC4 is part of a complex that also includes other proteins involved in RNA metabolism. Its function impacts gene expression by modulating the epigenetic marks on mRNA thereby altering the cellular response to different stimuli.

Pathways

ZCCHC4 contributes significantly to the epitranscriptome particularly affecting the m6A RNA methylation pathway. This modification is vital for processes like RNA splicing export and degradation. ZCCHC4 interacts with related proteins such as METTL3 and METTL14 which are core components of the m6A methyltransferase complex highlighting its integration within this regulatory network.

Associated diseases and disorders

Alterations in ZCCHC4 expression or function associate with certain cancers including liver cancer. Dysregulation of m6A modification in which ZCCHC4 is involved contributes to tumor progression and metastasis. Research has also linked ZCCHC4 to mental disorders with evidence suggesting that abnormal mRNA methylation affects neural gene expression. ZCCHC4's interactions with proteins like ALKBH5 an m6A demethylase further illustrate its involvement in the pathogenesis of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ZCCHC4 antibody [EPR20492] (ab211325), expandable thumbnail

    Western blot - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody works best on a semi-dry nitrocellulose membrane transfer system.

    These lysates were kindly provided by Prof Chuan He; University of Chicago.

    All lanes: Western blot - Anti-ZCCHC4 antibody [EPR20492] (ab211325) at 1/1000 dilution

    Lane 1: Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: ZCCHC4 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa

    Observed band size: 59 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (ab211325), expandable thumbnail

    Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

    ZCCHC4 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab211325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211325 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab211325 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab211325 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This antibody works best on a semi-dry nitrocellulose membrane transfer system.

    All lanes: Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

    Predicted band size: 59 kDa

  • Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (ab211325), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (ab211325), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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