AB211325

Anti-ZCCHC4 antibody [EPR20492]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ZCCHC4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

rRNA N(6)-adenosine-methyltransferase ZCCHC4, Zinc finger CCHC domain-containing protein 4, ZCCHC4

4 Images

Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

ZCCHC4 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab211325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211325 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab211325 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211325 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This antibody works best on a semi-dry nitrocellulose membrane transfer system.

All lanes:

Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (ab211325)

Predicted band size: 59 kDa

false

Supplier Data

Western blot - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Blocking/Dilution buffer : 5% NFDM/TBST.

This antibody works best on a semi-dry nitrocellulose membrane transfer system.

These lysates were kindly provided by Prof Chuan He; University of Chicago.

All lanes:

Western blot - Anti-ZCCHC4 antibody [EPR20492] (ab211325) at 1/1000 dilution

Lane 1:

Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

ZCCHC4 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20492

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This antibody works best on a semi-dry nitrocellulose membrane transfer system.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC4 also known as zinc finger CCHC-type containing 4 is a protein that functions as a methyltransferase specifically modifying mRNA by adding a methyl group at the N6 position of adenine. It has an estimated mass of approximately 66 kDa. Expression of ZCCHC4 is observed mainly in the nucleus of cells and it is found across various human tissues indicating its widespread functional importance.

Biological function summary

The methyltransferase activity of ZCCHC4 plays an important role in post-transcriptional regulation of mRNA influencing its stability and translation efficiency. ZCCHC4 is part of a complex that also includes other proteins involved in RNA metabolism. Its function impacts gene expression by modulating the epigenetic marks on mRNA thereby altering the cellular response to different stimuli.

Pathways

ZCCHC4 contributes significantly to the epitranscriptome particularly affecting the m6A RNA methylation pathway. This modification is vital for processes like RNA splicing export and degradation. ZCCHC4 interacts with related proteins such as METTL3 and METTL14 which are core components of the m6A methyltransferase complex highlighting its integration within this regulatory network.

Alterations in ZCCHC4 expression or function associate with certain cancers including liver cancer. Dysregulation of m6A modification in which ZCCHC4 is involved contributes to tumor progression and metastasis. Research has also linked ZCCHC4 to mental disorders with evidence suggesting that abnormal mRNA methylation affects neural gene expression. ZCCHC4's interactions with proteins like ALKBH5 an m6A demethylase further illustrate its involvement in the pathogenesis of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

rRNA N6-methyltransferase that specifically methylates the adenine in position 4220 of 28S rRNA (PubMed : 30531910, PubMed : 31328227, PubMed : 31695039, PubMed : 31799605). N6-methylation of adenine(4220) in 28S rRNA is required for translation (PubMed : 30531910, PubMed : 31799605).

See full target information rRNA N6-adenosine-methyltransferase ZCCHC4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:356 PubMed40629418

2025

DPSCs modulate synovial macrophage polarization and efferocytosis via PINK1/Parkin-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjin Ma,Xinyu Wang,Dalei Sun,Jiali Chen,Linyi Zhou,Kaiao Zou,Xinxin Ni,Hongting Jin,Jun Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZCCHC4 antibody [EPR20492]

Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Flow Cytometry (Intracellular) - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Western blot - Anti-ZCCHC4 antibody [EPR20492] (AB211325)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise