JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181152

Anti-ZCCHC8 antibody [EPR13612]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ZCCHC8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger CCHC domain-containing protein 8, TRAMP-like complex RNA-binding factor ZCCHC8, ZCCHC8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] (AB181152)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] (AB181152)

Immunofluorescence analysis of HeLa cells labeling ZCCHC8 with ab181152 at 1/250 dilution (left) and Dapi blue (right). Fixative : -20°C Acetone. Secondary : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.

Western blot - Anti-ZCCHC8 antibody [EPR13612] (AB181152)
  • WB

Supplier Data

Western blot - Anti-ZCCHC8 antibody [EPR13612] (AB181152)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ZCCHC8 antibody [EPR13612] (ab181152) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa

false

  • Carrier free

    Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13612

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB68739

Anti-ZCCHC8 antibody

Western blot - Anti-ZCCHC8 antibody (AB68739)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB70551

Anti-MTR4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (AB70551)

  • 4
  • 3
Primary Antibodies

AB75855

Anti-PABPN1 antibody [EP3000Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3000Y] (AB75855)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC8 or Zinc Finger CCHC-Type Containing 8 is a protein that stabilizes RNA and is involved in RNA decay playing a role in RNA metabolism. It is composed of approximately 92 kDa and is found mainly in the nucleus. ZCCHC8 is expressed in many tissues with notable levels in the brain and heart indicating its relevance in widely important cellular processes. Its function is not limited to RNA stabilisation as it also contributes to RNA processing and transport.
Biological function summary

ZCCHC8 participates in regulating gene expression by being a part of the nuclear exosome targeting (NEXT) complex which is responsible for the degradation of a variety of RNA species including long non-coding RNAs and promoter upstream transcripts. This involvement in RNA turnover indicates that ZCCHC8 helps maintain cellular RNA homeostasis contributing to the control of gene expression. The NEXT complex which includes other proteins like RBM7 and hMTR4 functions as a critical component in deciding which RNA substrates are selected for degradation reflecting the dynamic nature of RNA processing events in cells.

Pathways

ZCCHC8 is significant in the RNA surveillance pathway and RNA degradation pathway. Within these pathways ZCCHC8 contributes by ensuring defective or excess RNA molecules are adequately processed and degraded therefore preserving RNA quality and balance. It interacts with proteins such as RBM7 and the exosome complex emphasizing its integrative role in RNA metabolism and the importance of these interactions in safeguarding correct cellular function through efficient RNA management.

Alterations in ZCCHC8 function or expression levels have been associated with neurological disorders such as neurodevelopmental diseases and psychiatric conditions. Its connection with RNA metabolism suggests that disruptions in ZCCHC8 activity may affect neuronal function and development. Research has implicated the protein in disorders like schizophrenia where it likely interacts with RNA-binding proteins such as FMRP known to bind mRNA and is associated with fragile X syndrome. These associations highlight ZCCHC8's role in maintaining neural integrity and mental health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Scaffolding subunit of the trimeric nuclear exosome targeting (NEXT) complex that is involved in the surveillance and turnover of aberrant transcripts and non-coding RNAs (PubMed : 27871484). NEXT functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. May be involved in pre-mRNA splicing (Probable). It is required for 3'-end maturation of telomerase RNA component (TERC), TERC 3'-end targeting to the nuclear RNA exosome, and for telomerase function (PubMed : 31488579).
See full target information ZCCHC8
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 6: PubMed28984244

2017

YTHDC1 mediates nuclear export of N-methyladenosine methylated mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Ian A Roundtree,Guan-Zheng Luo,Zijie Zhang,Xiao Wang,Tao Zhou,Yiquang Cui,Jiahao Sha,Xingxu Huang,Laura Guerrero,Phil Xie,Emily He,Bin Shen,Chuan He
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com