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AB250380

Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZCCHC8 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

Zinc finger CCHC domain-containing protein 8, TRAMP-like complex RNA-binding factor ZCCHC8, ZCCHC8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free (AB250380)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free (AB250380)

This data was developed using ab181152, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling ZCCHC8 with ab181152 at 1/250 dilution (left) and Dapi blue (right). Fixative : -20°C Acetone. Secondary : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.

Western blot - Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free (AB250380)
  • WB

Supplier Data

Western blot - Anti-ZCCHC8 antibody [EPR13612] - BSA and Azide free (AB250380)

This data was developed using ab181152, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZCCHC8 antibody [EPR13612] (<a href='/en-us/products/primary-antibodies/zcchc8-antibody-epr13612-ab181152'>ab181152</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13612

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250380 is the carrier-free version of ab181152.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZCCHC8 or Zinc Finger CCHC-Type Containing 8 is a protein that stabilizes RNA and is involved in RNA decay playing a role in RNA metabolism. It is composed of approximately 92 kDa and is found mainly in the nucleus. ZCCHC8 is expressed in many tissues with notable levels in the brain and heart indicating its relevance in widely important cellular processes. Its function is not limited to RNA stabilisation as it also contributes to RNA processing and transport.
Biological function summary

ZCCHC8 participates in regulating gene expression by being a part of the nuclear exosome targeting (NEXT) complex which is responsible for the degradation of a variety of RNA species including long non-coding RNAs and promoter upstream transcripts. This involvement in RNA turnover indicates that ZCCHC8 helps maintain cellular RNA homeostasis contributing to the control of gene expression. The NEXT complex which includes other proteins like RBM7 and hMTR4 functions as a critical component in deciding which RNA substrates are selected for degradation reflecting the dynamic nature of RNA processing events in cells.

Pathways

ZCCHC8 is significant in the RNA surveillance pathway and RNA degradation pathway. Within these pathways ZCCHC8 contributes by ensuring defective or excess RNA molecules are adequately processed and degraded therefore preserving RNA quality and balance. It interacts with proteins such as RBM7 and the exosome complex emphasizing its integrative role in RNA metabolism and the importance of these interactions in safeguarding correct cellular function through efficient RNA management.

Alterations in ZCCHC8 function or expression levels have been associated with neurological disorders such as neurodevelopmental diseases and psychiatric conditions. Its connection with RNA metabolism suggests that disruptions in ZCCHC8 activity may affect neuronal function and development. Research has implicated the protein in disorders like schizophrenia where it likely interacts with RNA-binding proteins such as FMRP known to bind mRNA and is associated with fragile X syndrome. These associations highlight ZCCHC8's role in maintaining neural integrity and mental health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffolding subunit of the trimeric nuclear exosome targeting (NEXT) complex that is involved in the surveillance and turnover of aberrant transcripts and non-coding RNAs (PubMed : 27871484). NEXT functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. May be involved in pre-mRNA splicing (Probable). It is required for 3'-end maturation of telomerase RNA component (TERC), TERC 3'-end targeting to the nuclear RNA exosome, and for telomerase function (PubMed : 31488579).
See full target information ZCCHC8
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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