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AB14429

Anti-ZCWCC1 antibody

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(3 Publications)

Rabbit Polyclonal ZCWCC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MORC2 aa 600-700.

View Alternative Names

KIAA0852, ZCWCC1, MORC2, ATPase MORC2, MORC family CW-type zinc finger protein 2, Zinc finger CW-type coiled-coil domain protein 1

2 Images
Immunoprecipitation - Anti-ZCWCC1 antibody (AB14429)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZCWCC1 antibody (AB14429)

Immunoprecipitation of ZCWCC1 with ab14429 at 3 μg/mL.

Lane 1 : ab14429 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 2 : IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-ZCWCC1 antibody (ab14429)

Predicted band size: 118 kDa

false

Exposure time: 30s

Western blot - Anti-ZCWCC1 antibody (AB14429)
  • WB

Supplier Data

Western blot - Anti-ZCWCC1 antibody (AB14429)

All lanes:

Western blot - Anti-ZCWCC1 antibody (ab14429) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293 cell lysate at 50 µg

Predicted band size: 118 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MORC2 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6X9

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "3 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified using an epitope specific to ZCWCC1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ZCWCC1 also known as Zinc Finger CW-type Coiled-Coil Domain 1 is a molecular entity with a molecular mass of approximately 85 kDa. ZCWCC1 exists mainly in the nucleus of the cell where it plays a role in the regulation of gene expression. It exhibits a zinc finger motif which allows it to interact with DNA or RNA. Expression of ZCWCC1 is found across various tissue types but it is more prominent in rapidly dividing cells hinting at its involvement in cell cycle regulation.
Biological function summary

ZCWCC1 is implicated in chromatin modification and remodeling affecting how DNA is packed into chromatin. It forms part of a multiprotein complex that facilitates access to specific genetic regions by modifying histones which are proteins around which DNA winds. ZCWCC1 interacting with other proteins in the complex influences transcription and potentially gene silencing. Its function is integral in maintaining the structural configuration of chromatin and ensuring proper gene expression patterns during cellular processes.

Pathways

The activity of ZCWCC1 is integral to the chromatin organization pathway and the gene expression pathway. ZCWCC1 works in conjunction with proteins like histone deacetylases (HDACs) and histone methyltransferases which are vital for modifying histones and regulating gene transcription. These pathways ensure that genetic information is accurately transcribed and translated during various cellular processes including differentiation and proliferation.

ZCWCC1 has potential implications in cancer and neurodegenerative diseases. Aberrant expression or mutation of ZCWCC1 can lead to uncontrolled cell proliferation linking it to oncogenic processes in certain cancers. In neurodegenerative disorders dysregulation of chromatin remodeling where ZCWCC1 is involved is observed alongside factors like tau protein suggesting a complex interplay in neurodegeneration. Understanding ZCWCC1's role in these disease contexts may offer insights into novel therapeutic targets or biomarkers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for epigenetic silencing by the HUSH (human silencing hub) complex. Recruited by HUSH to target site in heterochromatin, the ATPase activity and homodimerization are critical for HUSH-mediated silencing (PubMed : 28581500, PubMed : 29440755, PubMed : 32693025). Represses germ cell-related genes and L1 retrotransposons in collaboration with SETDB1 and the HUSH complex, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed : 29211708). During DNA damage response, regulates chromatin remodeling through ATP hydrolysis. Upon DNA damage, is phosphorylated by PAK1, both colocalize to chromatin and induce H2AX expression. ATPase activity is required and dependent of phosphorylation by PAK1 and presence of DNA (PubMed : 23260667). Recruits histone deacetylases, such as HDAC4, to promoter regions, causing local histone H3 deacetylation and transcriptional repression of genes such as CA9 (PubMed : 20110259, PubMed : 20225202). Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation (PubMed : 24286864).
See full target information MORC2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 15:141-152 PubMed35035226

2022

Tumor-Infiltrating CD4 Central Memory T Cells Correlated with Favorable Prognosis in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wu,Tianyi Zhang,Haofeng Xiong,Liujun Zeng,Zijia Wang,Ying Peng,Weijun Chen,Xin Hu,Tong Su

International journal of molecular medicine 49: PubMed34913078

2021

Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Yunna Yang,Yipeng Dong,Cang Liu

FEBS letters 595:1289-1302 PubMed33626175

2021

The chromatin modifier MORC2 affects glucose metabolism by regulating the expression of lactate dehydrogenase A through a feed forward loop with c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Rohith Kumar Guddeti,Liz Thomas,Anbarasu Kannan,Prashanthi Karyala,Suresh B Pakala
View all publications
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