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AB126482

Anti-ZCWPW2 antibody

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(1 Publication)

Rabbit Polyclonal ZCWPW2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZCWPW2 aa 250 to C-terminus.

View Alternative Names

Zinc finger CW-type PWWP domain protein 2, ZCWPW2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZCWPW2 antibody (AB126482)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZCWPW2 antibody (AB126482)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, vesicles and cytoskeleton (microtubules). Recommended concentration of ab126482 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCWPW2 antibody (AB126482)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCWPW2 antibody (AB126482)

ab126482 at a 1/500 dilution staining ZCWPW2 in paraffin embedded Human adrenal gland tissue by immunohistochemistry.

Western blot - Anti-ZCWPW2 antibody (AB126482)
  • WB

Unknown

Western blot - Anti-ZCWPW2 antibody (AB126482)

All lanes:

Western blot - Anti-ZCWPW2 antibody (ab126482) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZCWPW2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q504Y3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZCWPW2 also known as Zinc Finger CW-Type PWWP Domain Protein 2 is a protein involved in epigenetic regulation. It has a molecular mass of around 153 kDa. ZCWPW2 plays a role in recognizing and binding to specific histone modifications which contributes to chromatin remodeling and gene expression regulation. Its expression occurs in various tissues including testis which suggests a role in reproductive processes and possibly in spermatogenesis.
Biological function summary

ZCWPW2 functions in modulating chromatin structure by interacting with modified histones. This protein is not known to be part of a large protein complex but it has connections with pathways that regulate gene transcription. By binding to trimethylated histone H3 lysine 36 (H3K36me3) ZCWPW2 facilitates the recruitment of other proteins necessary for transcriptional processes which has implications in cellular differentiation and development.

Pathways

ZCWPW2 participates in the gene expression and chromatin remodeling pathways. It interacts within the histone modification pathway where it associates with proteins like MLL3 and MLL4 which are important for methylation marks establishment. Such interactions indicate ZCWPW2’s involvement in transcriptional regulation by influencing chromatin accessibility ultimately affecting gene activity and tissue-specific gene expression patterns.

Abnormal function or expression of ZCWPW2 relates to cancer and neurodevelopmental disorders. Disruption in its activity can lead to improper gene expression patterns potentially resulting in carcinogenesis. Additionally the connection of epigenetic regulators like ZCWPW2 with cognitive dysfunction highlights its role in neurodevelopmental conditions. Through its chromatin-modifying activities ZCWPW2 may impact pathways involving SETD2 another histone methyltransferase which is also linked to tumorigenesis and cognitive disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3 (PubMed : 26933034). The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0 (PubMed : 26933034).
See full target information ZCWPW2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e22280 PubMed38034706

2023

Expression of the kidney anion exchanger 1 affects WNK4 and SPAK phosphorylation and results in claudin-4 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Rawad Lashhab,Grace Essuman,Maria Chavez-Canales,R Todd Alexander,Emmanuelle Cordat
View all publications
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Product promise

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