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AB235369

Anti-ZDHHC4/DC1 antibody

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(2 Publications)

Rabbit Polyclonal ZDHHC4/DC1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC4 aa 250 to C-terminus.

View Alternative Names

ZNF374, DC1, UNQ5787/PRO19576, ZDHHC4, Palmitoyltransferase ZDHHC4, Zinc finger DHHC domain-containing protein 4, Zinc finger protein 374, DHHC-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Paraffin-embedded human kidney tissue stained for ZDHHC4/DC1 using ab235369 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Paraffin-embedded human liver cancer tissue stained for ZDHHC4/DC1 using ab235369 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Paraffin-embedded human pancreatic tissue stained for ZDHHC4/DC1 using ab235369 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ZDHHC4/DC1 antibody (AB235369)
  • WB

Supplier Data

Western blot - Anti-ZDHHC4/DC1 antibody (AB235369)

All lanes:

Western blot - Anti-ZDHHC4/DC1 antibody (ab235369) at 1/500 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZDHHC4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPG8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZDHHC4 also known as DC1 is a palmitoyltransferase enzyme with a mass of approximately 35 kDa. It plays an important role in attaching palmitate a fatty acid to cysteine residues on target proteins which is an important post-translational modification. This modification helps in the trafficking stability and function of proteins within a cell. ZDHHC4 is expressed in various tissues including the brain and liver influencing diverse biological processes and cellular functions.
Biological function summary

This palmitoyltransferase influences protein-protein interactions by adding lipid moieties to key signaling molecules. It is part of the ZDHHC family of proteins which collaborate to modify a variety of substrates important for cellular signaling pathways. By palmitoylating proteins ZDHHC4 can regulate pathways involving signal transduction and vesicular trafficking affecting how signals are transmitted inside the cell and how proteins are transported to different cellular compartments.

Pathways

Research shows this enzyme acts within the signaling pathways such as the Wnt signaling and Ras signaling. In the Wnt pathway ZDHHC4's palmitoylation activity helps control the stability and localization of proteins important for signal propagation. Within the Ras signaling pathway it modifies Ras proteins which are related to cell growth and survival. These associations highlight its role in influencing cell behavior through complex signaling networks.

Mutations or dysregulation of ZDHHC4 have links to neurodegenerative diseases and cancer. For instance its activity impacts protein interactions that when impaired can contribute to Alzheimer's disease. In cancer ZDHHC4's altered palmitoylation activity can affect oncogenic signaling cascades contributing to tumor progression. It shows interaction with proteins such as beta-catenin in cancer and Beta-amyloid precursor in Alzheimer's connecting ZDHHC4 to these diseases at a molecular level.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Palmitoyltransferase that catalyzes the addition of palmitate onto protein substrates including the D(2) dopamine receptor DRD2, GSK3B or MAVS. Mediates GSK3B palmitoylation to prevent its AKT1-mediated phosphorylation leading to activation of the STAT3 signaling pathway (PubMed : 35606353). Catalyzes also MAVS palmitoylation which promotes its stabilization and activation by inhibiting 'Lys-48'- but facilitating 'Lys-63'-linked ubiquitination (PubMed : 38016475).
See full target information ZDHHC4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 11:28 PubMed35606353

2022

GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggang Zhao,Huihan Yu,Xiaoqing Fan,Wanxiang Niu,Junqi Fan,Suling Sun,Meiting Gong,Bing Zhao,Zhiyou Fang,Xueran Chen

Journal of inherited metabolic disease 45:635-656 PubMed35150145

2022

Ppt1-deficiency dysregulates lysosomal Ca homeostasis contributing to pathogenesis in a mouse model of CLN1 disease.

Applications

Unspecified application

Species

Unspecified reactive species

Avisek Mondal,Abhilash P Appu,Tamal Sadhukhan,Maria B Bagh,Rafael M Previde,Sriparna Sadhukhan,Stanko Stojilkovic,Aiyi Liu,Anil B Mukherjee
View all publications
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