Anti-ZDHHC4/DC1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC4/DC1 antibody (AB235369)

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas tissue labelling ZDHHC4/DC1 with ab235369 at a 1 : 50 dilution. The immunostaining was performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 min at RT. ab235369 was incubated at 4°C overnight. The primary was detected using a Goat anti-rabbit polymer IgG HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• WB

Supplier Data

Western blot - Anti-ZDHHC4/DC1 antibody (AB235369)

All lanes:

Western blot - Anti-ZDHHC4/DC1 antibody (ab235369) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

SY5Y whole cell lysate at 30 µg

Lane 4:

MCF7 whole cell lysate at 30 µg

Lane 5:

HT29 whole cell lysate at 30 µg

Lane 6:

Mouse Liver tissue lysate at 30 µg

Lane 7:

Rat Lung tissue lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 40 kDa

false

Exposure time: 120s