Rabbit Polyclonal ZDHHC9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZDHHC9.
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- Oncology letters 25:52022ZDHHC9 promotes colon tumor growth by inhibiting effector T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodan Chong,Lingxi Zhu,Dong Yu,Shasha Chen,Guang Wang,Qingzhuo Yu,Xianwei Ma,Jing Xu,Haitao Chen,Huazhang AnPubMed 36419754
- RSC chemical biology 2:192-2052021A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Yao,Chushu Li,Fang He,Teng Song,Jean-Philippe Brosseau,Huanbin Wang,Haojie Lu,Caiyun Fang,Hubing Shi,Jiang Lan,Jing-Yuan Fang,Jie XuPubMed 34458782
- Proceedings of the National Academy of Sciences of the United States of America 109:20473-82012Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach.Applications:WBReactive species:Unspecified reactive speciesXiaolu A Cambronne,Rongkun Shen,Paul L Auer,Richard H GoodmanPubMed 23184980
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZDHHC9. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y397
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as ADRB2, GSDMD, HRAS, NRAS and CGAS (PubMed:16000296, PubMed:27481942, PubMed:37802025, PubMed:38530158, PubMed:38599239). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS (PubMed:16000296). May have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and therefore regulate G protein-coupled receptor signaling (PubMed:27481942). Acts as a regulator of innate immunity by catalyzing palmitoylation of CGAS, thereby promoting CGAS homodimerization and cyclic GMP-AMP synthase activity (PubMed:37802025). Activates pyroptosis by catalyzing palmitoylation of gasdermin-D (GSDMD), thereby promoting membrane translocation and pore formation of GSDMD (PubMed:38530158, PubMed:38599239). (Microbial infection) Through a sequential action with ZDHHC20, rapidly and efficiently palmitoylates SARS coronavirus-2/SARS-CoV-2 spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell.
Alternative names
CXorf11, ZDHHC10, ZNF379, ZNF380, CGI-89, UNQ261/PRO298, ZDHHC9, Palmitoyltransferase ZDHHC9, Zinc finger DHHC domain-containing protein 9, Zinc finger protein 379, Zinc finger protein 380, DHHC-9, DHHC9
Recommended products
Rabbit Polyclonal ZDHHC9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZDHHC9.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZDHHC9. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y397
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZDHHC9 also known as DHHC9 is a protein that functions as a palmitoyltransferase catalyzing the addition of palmitate a fatty acid to cysteine residues on substrate proteins. This post-translational modification known as S-palmitoylation regulates protein stability localization and interactions. ZDHHC9 has a molecular weight of approximately 39 kDa and is widely expressed in various tissues including the brain and heart. The protein localizes to the Golgi apparatus and affects the membrane association of its substrate proteins.
- Biological function summary
ZDHHC9 influences cellular signaling and protein trafficking. It forms a complex with GCP16 another subunit enhancing its palmitoylation activity. ZDHHC9 regulates the palmitoylation of proteins involved in signaling pathways such as Ras proteins. This modification affects Ras proteins' membrane localization and function impacting cellular communication and signaling events. By modulating these pathways ZDHHC9 plays a part in maintaining normal cell physiology.
- Pathways
ZDHHC9 modifies signal transduction pathways like the MAPK/ERK and the NF-κB pathways. The palmitoylated proteins especially H-Ras and N-Ras are integral to these signaling pathways that control cell growth differentiation and survival. The enzyme’s activity affects the dynamic regulation of these pathways influencing how cells respond to external stimuli. By interacting with these Ras proteins ZDHHC9 indirectly impacts pathways that regulate essential cellular processes.
- Associated diseases and disorders
Mutations or dysregulation of ZDHHC9 have associations with mental retardation and X-linked cognitive impairment. These conditions relate to altered Ras signaling where ZDHHC9 mutations impact normal Ras palmitoylation and function. Moreover defects in this protein have connections to developmental abnormalities due to disruptions in signal transduction during brain development. Understanding ZDHHC9's interactions with proteins like Ras provides insight into these complex disorders and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ZDHHC9 antibody (ab74504)
All lanes: Western blot - Anti-ZDHHC9 antibody (ab74504) at 1/500 dilution
Lane 1: extracts from COLO cells at 30 µg
Lane 2: extracts from COLO cells at 30 µg with immunising peptide
Predicted band size: 41 kDa
Observed band size: 26 kDa, 34 kDa, 41 kDa, 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZDHHC9 antibody (ab74504)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74504. Left: untreated sample. Right: sample treated with immunising peptide.
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