AB124512

Anti-ZEB1 antibody

Name: Anti-ZEB1 antibody (ab124512) | Abcam
Brand: Abcam Limited
SKU: AB124512
Price: 670 USD
Availability: InStock

(1 Review)

(45 Publications)

Rabbit Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1.

View Alternative Names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB124512)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB124512)

Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20μg/ml.

Supplier Data

Immunocytochemistry - Anti-ZEB1 antibody (AB124512)

Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.

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Immunocytochemistry - Anti-ZEB1 antibody (AB124512)

Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.

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Western blot - Anti-ZEB1 antibody (AB124512)

All lanes:

Western blot - Anti-ZEB1 antibody (ab124512) at 1 µg/mL

All lanes:

HeLa cell lysate at 15 µg

Predicted band size: 124 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ZEB1. The exact immunogen used to generate this antibody is proprietary information.

P37275

Specificity

ab124512 is predicted not to crossreact with ZEB2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.

Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).

See full target information ZEB1

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107598 PubMed39059495

2024

N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Cai,Jiawang Zhou,Abai Xu,Jinchang Huang,Haisheng Zhang,Guoyou Xie,Ke Zhong,You Wu,Pengfei Ye,Hongsheng Wang,Hongxin Niu

NPJ precision oncology 8:79 PubMed38548861

2024

ELAVL2 loss promotes aggressive mesenchymal transition in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yona Kim,Ji Hyeon You,Yeonjoo Ryu,Gyuri Park,Urim Lee,Hyo Eun Moon,Hye Ran Park,Chang W Song,Ja-Lok Ku,Sung-Hye Park,Sun Ha Paek

Neurobiology of disease 178:106030 PubMed36736597

2023

Identification of a novel pathway in sporadic Amyotrophic Lateral Sclerosis mediated by the long non-coding RNA ZEB1-AS1.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Rey,Erika Maghraby,Letizia Messa,Letizia Esposito,Bianca Barzaghini,Cecilia Pandini,Matteo Bordoni,Stella Gagliardi,Luca Diamanti,Manuela Teresa Raimondi,Massimiliano Mazza,Gianvincenzo Zuccotti,Stephana Carelli,Cristina Cereda

Arteriosclerosis, thrombosis, and vascular biology 42:1254-1261 PubMed36004642

2022

Inhibition of the Renin-Angiotensin System Fails to Suppress β-Aminopropionitrile-Induced Thoracic Aortopathy in Mice-Brief Report.

Applications

Unspecified application

Species

Mouse

Hisashi Sawada,Satoko Ohno-Urabe,Dien Ye,Michael K Franklin,Jessica J Moorleghen,Deborah A Howatt,Adam E Mullick,Alan Daugherty,Hong S Lu

Cell death discovery 8:271 PubMed35614051

2022

GPR110 promotes progression and metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Jung Nam,Yeon-Ju Kim,Jae-Hyeok Kang,Su-Jae Lee

Frontiers in molecular biosciences 9:807324 PubMed35480877

2022

Nrf2 Modulates the Hybrid Epithelial/Mesenchymal Phenotype and Notch Signaling During Collective Cancer Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel A Vilchez Mercedes,Federico Bocci,Mona Ahmed,Ian Eder,Ninghao Zhu,Herbert Levine,José N Onuchic,Mohit Kumar Jolly,Pak Kin Wong

Cell reports 37:109996 PubMed34818544

2021

Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Ju Kim,Yi Zhao,Jae Kyung Myung,Joo Mi Yi,Min-Jung Kim,Su-Jae Lee

Experimental and therapeutic medicine 22:1390 PubMed34650638

2021

Monensin suppresses cell proliferation and invasion in ovarian cancer by enhancing MEK1 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shujuan Yao,Wen Wang,Bin Zhou,Xiujuan Cui,Hui Yang,Shiqian Zhang

Frontiers in cell and developmental biology 9:684819 PubMed34222253

2021

Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer Regulating Tropomyosin 4.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Zhao,Mei Jiang,Yu Teng,Jie Li,Zhefeng Li,Wende Hao,Hongyu Zhao,Chenghong Yin,Wentao Yue

Molecular medicine reports 23: PubMed33846788

2021

Zinc‑finger E‑box‑binding homeobox 1 alleviates acute kidney injury by activating autophagy and the AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dehua Sun,Xiaohua Liu,Lijuan Zhu,Bin Zhang

View all publications

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