Rabbit Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1.
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- Arteriosclerosis, thrombosis, and vascular biology 42:1254-12612022Inhibition of the Renin-Angiotensin System Fails to Suppress β-Aminopropionitrile-Induced Thoracic Aortopathy in Mice-Brief Report.Applications:Unspecified applicationReactive species:MouseHisashi Sawada,Satoko Ohno-Urabe,Dien Ye,Michael K Franklin,Jessica J Moorleghen,Deborah A Howatt,Adam E Mullick,Alan Daugherty,Hong S LuPubMed 36004642
- Cell death discovery 8:2712022GPR110 promotes progression and metastasis of triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Jung Nam,Yeon-Ju Kim,Jae-Hyeok Kang,Su-Jae LeePubMed 35614051
- Cell reports 37:1099962021Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeon-Ju Kim,Yi Zhao,Jae Kyung Myung,Joo Mi Yi,Min-Jung Kim,Su-Jae LeePubMed 34818544
- Experimental and therapeutic medicine 22:13902021Monensin suppresses cell proliferation and invasion in ovarian cancer by enhancing MEK1 SUMOylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShujuan Yao,Wen Wang,Bin Zhou,Xiujuan Cui,Hui Yang,Shiqian ZhangPubMed 34650638
- Frontiers in cell and developmental biology 9:6848192021Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer Regulating Tropomyosin 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoting Zhao,Mei Jiang,Yu Teng,Jie Li,Zhefeng Li,Wende Hao,Hongyu Zhao,Chenghong Yin,Wentao YuePubMed 34222253
- American journal of translational research 13:1037-10502021lncRNA SNHG17 promotes pancreatic carcinoma progression via cross-talking with miR-942.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangchao Zhao,Jinhua Ye,Yifan Lu,Changjie Sun,Xiaxing DengPubMed 33841638
- PloS one 15:e02411402020Potential association of LOXL1 with peritoneal dissemination in gastric cancer possibly via promotion of EMT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingjiang Hu et. al.PubMed 33095806
- Life sciences 257:1180822020circ_KIAA1429 accelerates hepatocellular carcinoma advancement through the mechanism of mA-YTHDF3-Zeb1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaoyu Wang et. al.PubMed 32653519
- Molecular medicine reports 22:1382-13902020Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Zhou et. al.PubMed 32468048
- Cancer management and research 12:3223-32352020WD Repeat Domain 5 Promotes Invasion, Metastasis and Tumor Growth in Glioma Through Up-Regulated Zinc Finger E-Box Binding Homeobox 1 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Dai et. al.PubMed 32440219
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZEB1. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Associated Products
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Target data
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Recommended products
Rabbit Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZEB1. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
- Purification technique
- Affinity purification Immunogen
- Specificity
ab124512 is predicted not to crossreact with ZEB2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
- Biological function summary
ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.
- Pathways
ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.
- Associated diseases and disorders
The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry - Anti-ZEB1 antibody (ab124512)
Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.
Western blot - Anti-ZEB1 antibody (ab124512)
All lanes: Western blot - Anti-ZEB1 antibody (ab124512) at 1 µg/mL
All lanes: HeLa cell lysate at 15 µg
Predicted band size: 124 kDa
Immunocytochemistry - Anti-ZEB1 antibody (ab124512)
Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab124512)
Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20μg/ml.
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