Anti-ZEB1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB155249)

Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ZEB1 at nucleus (green) with ab155249 at 1/500 dilution. Red : Phalloidin, a cytoskeleton marker, at 1/100 dilution.

Scale bar = 10 μm.

• IP

Supplier Data

Immunoprecipitation - Anti-ZEB1 antibody (AB155249)

IP Sample : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

A - 40 μg of HEK-293T whole cell lysate.

B - Control with 2 μg of preimmune rabbit IgG.

C - Immunoprecipitation of ZEB1 protein by 2 μg of ab155249.

The immunoprecipitated ZEB1 protein was detected by ab155249 diluted at 1 : 1000, followed by anti-rabbit IgG.

7.5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-ZEB1 antibody (ab155249)

Predicted band size: 124 kDa

false

• ChIP

Supplier Data

ChIP - Anti-ZEB1 antibody (AB155249)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab155249. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48) : 19204-9.

• WB

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. The observed M.W. is based on the publication : PMID : 19706487.

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/1000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/500 dilution

Lane 1:

Non-transfected (-) HeLa whole cell extracts at 30 µg

Lane 2:

Transfected (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

true

• WB

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

5% SDS-PAGE

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-ZEB1 antibody (AB155249)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZEB1 antibody (ab155249) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false