Rabbit Polyclonal ZEB1 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1 aa 250-600.
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- Nature communications 14:59582023Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPingting Ying,Can Chen,Zequn Lu,Shuoni Chen,Ming Zhang,Yimin Cai,Fuwei Zhang,Jinyu Huang,Linyun Fan,Caibo Ning,Yanmin Li,Wenzhuo Wang,Hui Geng,Yizhuo Liu,Wen Tian,Zhiyong Yang,Jiuyang Liu,Chaoqun Huang,Xiaojun Yang,Bin Xu,Heng Li,Xu Zhu,Ni Li,Bin Li,Yongchang Wei,Ying Zhu,Jianbo Tian,Xiaoping MiaoPubMed 37749132
- The journal of gene medicine 25:e34762023METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaifeng Ni,Chengxian Liang,Zhen Zhou,Bo Jiang,Yong Li,Haiqiong Shang,Xiaoyu YuPubMed 36735630
- International journal of biological sciences 18:2186-22012022SENP1 promotes triple-negative breast cancer invasion and metastasis via enhancing CSN5 transcription mediated by GATA1 deSUMOylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongchang Gao,Rongrong Wang,Jianjing Liu,Ke Zhao,Xiaolong Qian,Xianghui He,Hong LiuPubMed 35342335
- Cell cycle (Georgetown, Tex.) 21:851-8732022LncRNA LINC01315 silencing modulates cancer stem cell properties and epithelial-to-mesenchymal transition in colorectal cancer via miR-484/DLK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouran Li,Wei Wang,Minna Wu,Ping Zhu,Zailong Zhou,Yuxia Gong,Yunfei GuPubMed 35156543
- Frontiers in genetics 12:6500902021Downregulated F-Box/LRR-Repeat Protein 7 Facilitates Pancreatic Cancer Metastasis by Regulating Snail1 for Proteasomal Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Tang,Meng Ji,Xing Liang,Danlei Chen,Anan Liu,Guang Yang,Ligang Shi,Zhiping Fu,Chenghao ShaoPubMed 34249081
- OncoTargets and therapy 13:9281-92892020Downregulation of PFTK1 Inhibits Migration and Invasion of Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Jiang et. al.PubMed 33061417
- BMC molecular and cell biology 21:652020Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianzhou Liu et. al.PubMed 32928102
- BioMed research international 2020:79072692020Mir-30b-5p Promotes Proliferation, Migration, and Invasion of Breast Cancer Cells via Targeting ASPP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianqi Wu et. al.PubMed 32420372
- Cancers 12:2020The NANOG Transcription Factor Induces Type 2 Deiodinase Expression and Regulates the Intracellular Activation of Thyroid Hormone in Keratinocyte Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnarita Nappi et. al.PubMed 32197405
- Biochimica et biophysica acta. Molecular basis of 1865:2563-25752019Protein arginine methyltranferase-1 induces ER stress and epithelial-mesenchymal transition in renal tubular epithelial cells and contributes to diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin-Yin Chen et. al.PubMed 31199999
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZEB1 aa 250-600. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
Reactivity data
Select an application| IP | ChIP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Recommended products
Rabbit Polyclonal ZEB1 antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1 aa 250-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZEB1 aa 250-600. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
- Biological function summary
ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.
- Pathways
ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.
- Associated diseases and disorders
The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
ChIP - Anti-ZEB1 antibody (ab155249)
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or ab155249. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9.
Western blot - Anti-ZEB1 antibody (ab155249)
5% SDS-PAGE
All lanes: Western blot - Anti-ZEB1 antibody (ab155249) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa
Immunoprecipitation - Anti-ZEB1 antibody (ab155249)
IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
A - 40 μg of HEK-293T whole cell lysate.
B - Control with 2 μg of preimmune rabbit IgG.
C - Immunoprecipitation of ZEB1 protein by 2 μg of ab155249.
The immunoprecipitated ZEB1 protein was detected by ab155249 diluted at 1:1000, followed by anti-rabbit IgG.
7.5% SDS-PAGE
All lanes: Immunoprecipitation - Anti-ZEB1 antibody (ab155249)
Predicted band size: 124 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab155249)
Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ZEB1 at nucleus (green) with ab155249 at 1/500 dilution. Red: Phalloidin, a cytoskeleton marker, at 1/100 dilution.
Scale bar = 10 μm.
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