Rabbit Polyclonal ZEB1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus.
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- Journal of translational medicine 21:8822023GATA6-AS1 suppresses epithelial-mesenchymal transition of pancreatic cancer under hypoxia through regulating SNAI1 mRNA stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhui Zhou,Xinyi Zhou,Qiwen Ben,Ningning Liu,Jiahui Wang,Yongpeng Zhai,Yichen Bao,Lin ZhouPubMed 38057853
- Journal of cellular and molecular medicine 24:13196-132072020Remifentanil up-regulates HIF1α expression to ameliorate hepatic ischaemia/reperfusion injury via the ZEB1/LIF axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongsheng Zhou,Shuang Li,Xiaopeng Mei,Tao Jiang,Qiang WangPubMed 32996684
- Cell death & disease 10:9322019CircRNA hsa_circ_0074834 promotes the osteogenesis-angiogenesis coupling process in bone mesenchymal stem cells (BMSCs) by acting as a ceRNA for miR-942-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengxiao Ouyang et. al.PubMed 31804461
- Journal of experimental & clinical cancer research 38:3662019A central role for MeCP2 in the epigenetic repression of miR-200c during epithelial-to-mesenchymal transition of glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErbao Bian et. al.PubMed 31429770
- Life sciences 233:1166922019Circular RNA hsa_circ_0007059 restrains proliferation and epithelial-mesenchymal transition in lung cancer cells via inhibiting microRNA-378.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShunji Gao et. al.PubMed 31351967
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Recommended products
Rabbit Polyclonal ZEB1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245283 was affinity purified using an epitope specific to ZEB1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
- Biological function summary
ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.
- Pathways
ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.
- Associated diseases and disorders
The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-ZEB1 antibody (ab245283)
ZEB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245283 at 3 μg per reaction. Western blot was performed from the immunoprecipitate using ab245283 at 0.4 μg/ml.
Lane 1: ab245283 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
All lanes: Immunoprecipitation - Anti-ZEB1 antibody (ab245283)
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30s
Western blot - Anti-ZEB1 antibody (ab245283)
Lysates prepared in NETN buffer.
All lanes: Western blot - Anti-ZEB1 antibody (ab245283) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg
Predicted band size: 124 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody (ab245283)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ZEB1 using ab245283 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody (ab245283)
Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for ZEB1 using ab245283 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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