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AB327209

Anti-ZEB1 Antibody

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Rabbit Polyclonal ZEB1 antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1.

View Alternative Names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemical staining of human cerebral cortex using ab327209 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in glial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemical staining of human liver using ab327209 at 1 : 500–1 : 1000 dilution, showing no nuclear positivity in hepatocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemical staining of human glioma using ab327209 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in tumor cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemical staining of human endometrium using ab327209 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in stromal cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemical staining of human prostate using ab327209 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in smooth muscle cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 Antibody (AB327209)

Immunohistochemistry analysis in human cerebral cortex and liver tissues using ab327209 at 1 : 500–1 : 1000 dilution. Corresponding ZEB1 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 Antibody (AB327209)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 Antibody (AB327209)

Immunofluorescent staining of human cell line U-2 OS using ab327209 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-ZEB1 Antibody (AB327209)
  • ChIP

Supplier Data

ChIP - Anti-ZEB1 Antibody (AB327209)

ChIP-Exo-Seq composite graph for ab327209 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZEB1.

P37275

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
See full target information ZEB1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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