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AB81972

Anti-ZEB1 antibody

1

(2 Reviews)

|

(10 Publications)

Goat Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 300-350.

View Alternative Names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB81972)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (AB81972)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot - Anti-ZEB1 antibody (AB81972)
  • WB

Unknown

Western blot - Anti-ZEB1 antibody (AB81972)

All lanes:

Western blot - Anti-ZEB1 antibody (ab81972) at 0.05 µg/mL

All lanes:

Mouse heart tissue lysate in ripa buffer at 35 µg

Predicted band size: 124 kDa

Observed band size: 100 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ZEB1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P37275

Specificity

ab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
See full target information ZEB1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:564-575 PubMed38449391

2024

The deubiquitinase BRCC3 increases the stability of ZEB1 and promotes the proliferation and metastasis of triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qidi Huang,Shurong Zheng,Huayan Gu,Zhi Yang,Yiqiao Lu,Xia Yu,Guilong Guo

Oncology reports 50: PubMed37859611

2023

PRMT1 accelerates cell proliferation, migration, and tumor growth by upregulating ZEB1/H4R3me2as in thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guoli Feng,Changju Chen,Yi Luo

Journal of nanobiotechnology 18:171 PubMed33218341

2020

Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ni,Yihai Liu,Lina Kang,Lian Wang,Zhonglin Han,Kun Wang,Biao Xu,Rong Gu

Cell death & disease 11:525 PubMed32655137

2020

LncRNA SNHG15 regulates EGFR-TKI acquired resistance in lung adenocarcinoma through sponging miR-451 to upregulate MDR-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayuan Huang,Banzhou Pan,Guohao Xia,Jingni Zhu,Chenchen Li,Jifeng Feng

The Kaohsiung journal of medical sciences 36:265-273 PubMed31889432

2020

MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Shi-Ying Ren,Yan Qu,Cui Liu,Yi Zhang,Xiang Qing Li,Hong Ma

Aging 11:10409-10421 PubMed31739286

2019

The anti-inflammation, anti-oxidative and anti-fibrosis properties of swertiamarin in cigarette smoke exposure-induced prostate dysfunction in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglou Chen,Jianhua Liu,Yongfang Lei,Min Liu

International journal of clinical and experimental 12:4066-4076 PubMed31933802

2019

Hsa_circ_0057481 promotes laryngeal cancer proliferation and migration by modulating the miR-200c/ZEB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dehui Fu,Yongwang Huang,Ming Gao

Journal of cellular physiology 234:17639-17648 PubMed30854680

2019

microRNA-203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2.

Applications

Unspecified application

Species

Unspecified reactive species

Haochuan Liu,Bing Chen,Yi Li

Oncogene 37:6316-6326 PubMed30042416

2018

Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojia Huang,Xinhua Xie,Peng Liu,Lu Yang,Bo Chen,Cailu Song,Hailin Tang,Xiaoming Xie

PloS one 11:e0148774 PubMed26882471

2016

ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer.

Applications

WB

Species

Unspecified reactive species

Lingjia Liu,Qi Tong,Shuo Liu,Jianlin Cui,Quansheng Zhang,Wei Sun,Shuang Yang
View all publications
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