Goat Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 300-350.
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- Oncology reports 50:2023PRMT1 accelerates cell proliferation, migration, and tumor growth by upregulating ZEB1/H4R3me2as in thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoli Feng,Changju Chen,Yi LuoPubMed 37859611
- Journal of nanobiotechnology 18:1712020Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Ni et. al.PubMed 33218341
- Cell death & disease 11:5252020LncRNA SNHG15 regulates EGFR-TKI acquired resistance in lung adenocarcinoma through sponging miR-451 to upregulate MDR-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayuan Huang et. al.PubMed 32655137
- The Kaohsiung journal of medical sciences 36:265-2732020MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Liu,Shi-Ying Ren,Yan Qu,Cui Liu,Yi Zhang,Xiang Qing Li,Hong MaPubMed 31889432
- Aging 11:10409-104212019The anti-inflammation, anti-oxidative and anti-fibrosis properties of swertiamarin in cigarette smoke exposure-induced prostate dysfunction in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinglou Chen et. al.PubMed 31739286
- International journal of clinical and experimental 12:4066-40762019Hsa_circ_0057481 promotes laryngeal cancer proliferation and migration by modulating the miR-200c/ZEB1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDehui Fu et. al.PubMed 31933802
- Oncogene 37:6316-63262018Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojia Huang et. al.PubMed 30042416
- PloS one 11:e01487742016ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZEB1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Hamster | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.05000-0.20000 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Recommended products
Goat Polyclonal ZEB1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZEB1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
- Purification technique
- Affinity purification Immunogen
- Specificity
ab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
- Biological function summary
ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.
- Pathways
ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.
- Associated diseases and disorders
The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ZEB1 antibody (ab81972)
All lanes: Western blot - Anti-ZEB1 antibody (ab81972) at 0.05 µg/mL
All lanes: Mouse heart tissue lysate in ripa buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Observed band size: 100 kDa
Exposure time: 1hr
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody (ab81972)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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