AB87280

Anti-ZEB1 antibody

Name: Anti-ZEB1 antibody (ab87280) | Abcam
Brand: Abcam Limited
SKU: AB87280
Rating: 4 (5 reviews)

(5 Reviews)

(21 Publications)

Rabbit Polyclonal ZEB1 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus.

View Alternative Names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody (AB87280)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody (AB87280)

ab87280, at 1/250 dilution, staining ZEB1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry. Detection : DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody (AB87280)

ab87280, at 1/250 dilution, staining ZEB1 in formalin fixed, paraffin embedded mouse teratoma tissue by Immunohistochemistry. Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ZEB1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P37275

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Reactivity data

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Product details

Concentration is optimized for IHC and not determined

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab87280 was affinity purified using an epitope specific to ZEB1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.

Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).

See full target information ZEB1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6011 PubMed40593646

2025

Adipocyte-specific Zeb1 downregulation remodels the tumor-associated adipose microenvironment to facilitate female breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Cao,Wei Sun,Xiao Chen,Lei Liu,Shaorong Zhao,Jingjing Liu,Yang Ou,Min Guo,Chunchun Qi,Zhaoxian Li,Jie Shi,Yuxin Liu,Qiuying Shuai,Siyu Zuo,Huayu Hu,Tianwen Yu,Yanjing Wang,Mengdan Feng,Jianying Lv,Hang Wang,Peiqing Sun,Jin Zhang,Longlong Wang,Yi Shi,Shuang Yang

American journal of cancer research 14:4378-4397 PubMed39417185

2024

The Zeb1-Cxcl1 axis impairs the antitumor immune response by inducing M2 macrophage polarization in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Ou,Hui-Min Jiang,Yan-Jing Wang,Qiu-Ying Shuai,Li-Xia Cao,Min Guo,Chun-Chun Qi,Zhao-Xian Li,Jie Shi,Hua-Yu Hu,Yu-Xin Liu,Si-Yu Zuo,Xiao Chen,Meng-Dan Feng,Yi Shi,Pei-Qing Sun,Hang Wang,Shuang Yang

Medicine 102:e33834 PubMed37335710

2023

Aberrant expression of UBE2C in endometrial cancer and its correlation to epithelial mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Xueting Li,Yingying Gong,Danli Du,Huilei Chen,Lei Liu,Zenong Cheng

Journal of biochemical and molecular toxicology 37:e23373 PubMed37253097

2023

CUL4A-mediated ZEB1/microRNA-340-5p/HMGB1 axis promotes the development of osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongliang Chen,Qiang Zheng,You Lv,Zhongfeng Yang,Qin Fu

Cell death & disease 14:158 PubMed36841807

2023

DAPL1 prevents epithelial-mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Ma,Shuxian Han,Youjia Liu,Yu Chen,Pingping Li,Xiaoyan Liu,Lifu Chang,Ying-Ao Chen,Feng Chen,Qiang Hou,Ling Hou

Journal of ovarian research 14:33 PubMed33583413

2021

Survival and biomarker analysis for ovarian mucinous carcinoma according to invasive patterns: retrospective analysis and review literature.

Applications

Unspecified application

Species

Unspecified reactive species

Taira Hada,Morikazu Miyamoto,Hiroki Ishibashi,Hiroko Matsuura,Takahiro Sakamoto,Soichiro Kakimoto,Hideki Iwahashi,Hitoshi Tsuda,Masashi Takano

Medicine 100:e24472 PubMed33530259

2021

Low PRRX1 expression and high ZEB1 expression are significantly correlated with epithelial-mesenchymal transition and tumor angiogenesis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixue Yang,Yuanqun Liu,Yufei Wang,Xiaolin Wang,Hongfei Ci,Chao Song,Shiwu Wu

Cancer cell 38:212-228.e13 PubMed32707078

2020

Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay M LaFave,Vinay K Kartha,Sai Ma,Kevin Meli,Isabella Del Priore,Caleb Lareau,Santiago Naranjo,Peter M K Westcott,Fabiana M Duarte,Venkat Sankar,Zachary Chiang,Alison Brack,Travis Law,Haley Hauck,Annalisa Okimoto,Aviv Regev,Jason D Buenrostro,Tyler Jacks

Signal transduction and targeted therapy 5:25 PubMed32296027

2020

CDK4/6 inhibition blocks cancer metastasis through a USP51-ZEB1-dependent deubiquitination mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Jianjun Li,Yang Ou,Guang Yang,Kaiyuan Deng,Qiong Wang,Zhaoyang Wang,Wenhao Wang,Quansheng Zhang,Hang Wang,Wei Sun,Peiqing Sun,Shuang Yang

Frontiers in molecular biosciences 7:22 PubMed32185181

2020

Combined Snail and E-cadherin Predicts Overall Survival of Cervical Carcinoma Patients: Comparison Among Various Epithelial-Mesenchymal Transition Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejun Tian,Ping Qi,Qian Niu,Xuemei Hu

View all publications

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