Anti-ZEB1 antibody [BLR102H]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-ZEB1 antibody [BLR102H] (AB276129)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Zeb1 with ab276129 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

• IP

Supplier Data

Immunoprecipitation - Anti-ZEB1 antibody [BLR102H] (AB276129)

ZEB1 was immunoprecipitated from 20 μl/mg HEK-293T whole cell lysate, prepared using NETN lysis buffer. ab276129 was used for IP at 1 : 1000 (Lane 1). Control IgG (Lane 2).

Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZEB1 antibody [BLR102H] (ab276129)

Predicted band size: 124 kDa

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• WB

Supplier Data

Western blot - Anti-ZEB1 antibody [BLR102H] (AB276129)

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution

Lane 1:

GaMG whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 50 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

RKO whole cell lysate at 50 µg

Lane 5:

MCF-7 whole cell lysate at 50 µg

Lane 6:

A549 whole cell lysate at 50 µg

Lane 7:

K562 whole cell lysate at 50 µg

Lane 8:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 124 kDa

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• WB

Supplier Data

Western blot - Anti-ZEB1 antibody [BLR102H] (AB276129)

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution

Lane 1:

C2C12 whole cell lysate at 50 µg

Lane 2:

70Z/3 whole cell lysate at 50 µg

Lane 3:

BW5147.3 whole cell lysate at 50 µg

Lane 4:

CTLL-2 whole cell lysate at 50 µg

Lane 5:

EL4 whole cell lysate at 50 µg

Lane 6:

RenCa whole cell lysate at 50 µg

Lane 7:

NIH/3T3 whole cell lysate at 50 µg

Lane 8:

TCMK-1 whole cell lysate at 50 µg

Lane 9:

A20 whole cell lysate at 50 µg

Lane 10:

Ba/F3 whole cell lysate at 50 µg

Lane 11:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 124 kDa

false

• WB

CiteAb

Western blot - Anti-ZEB1 antibody [BLR102H] (AB276129)

ZEB1 western blot using anti-ZEB1 antibody [BLR102H] ab276129. Publication image and figure legend from You, W., Azuma, K., et al., 2024, Sci Rep, PubMed 39020017.

ab276129 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab276129 please see the product overview.

Fer-1 suppresses EMT-induced upregulation in the expressions of fibronectin and ZEB1 in ARPE-19 cells. (A) Representative western blot images of fibronectin (FN1), ZEB1, and inner control GAPDH. (B) Quantification adjusted to GAPDH of the western blot images, and comparisons among the four groups. (C) Relative mRNA levels of fibronectin and ZEB1 in the four groups. Data is presented by mean ± SD; n = 3 per group; **P ≤ 0.01, ****P ≤ 0.0001, ns not significant.

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