Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)

Rabbit Recombinant Monoclonal ZEB1 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 750-800.

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Images

Flow Cytometry - Anti-ZEB1 antibody [BLR217K] - BSA free (AB314092), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human ZEB1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link P37275

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Expected
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information ZEB1

Function

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).

Alternative names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

Recommended products

Rabbit Recombinant Monoclonal ZEB1 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ZEB1 aa 750-800.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human ZEB1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
Clone number: BLR217K
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.

Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

Associated diseases and disorders

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.

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9 product images

Flow Cytometry - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of human Zeb1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Zeb1 recombinant monoclonal [BLR217K] (A700-217) or isotype control (unshaded). Secondary: 650-conjugated goat anti-rabbit IgG.
Flow Cytometry - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of mouse Zeb1 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-Zeb1 recombinant monoclonal [BLR217K] (A700-217) or isotype control (unshaded). Secondary: 650-conjugated goat anti-rabbit IgG.
Western blot - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Samples: Whole cell lysate (10 µg) from Jurkat, HeLa, RKO, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR217K] (A700-217) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
All lanes: Western blot - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092) at 1/1000 dilution
Lane 1: Jurkat Whole cell lysate at 10 µg
Lane 2: HeLa Whole cell lysate at 10 µg
Lane 3: RKO Whole cell lysate at 10 µg
Lane 4: Hep-G2 Whole cell lysate at 10 µg
Lane 5: HEK293T Whole cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit antiZEB1 recombinant monoclonal antibody [BLR217K] (A700-217) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.
All lanes: Western blot - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092) at 1/1000 dilution
Lane 1: NIH 3T3 Whole cell lysate at 10 µg
Lane 2: CT26 Whole cell lysate at 10 µg
Lane 3: CH27 Whole cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR217K] (A700-217) used for IP at 6 µl/mg lysate. ZEB1 was also immunoprecipitated by a second antibody against a different epitope of ZEB1 (A301-922A). For blotting immunoprecipitated ZEB1, A700-217 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of mouse ZEB1 by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR217K] (A700-217-1). Secondary: HRPconjugated goat anti-rabbit IgG (A120-501P).
Immunocytochemistry - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of human ZEB1 by immunocytochemistry. Sample:FFPE section of RKO cells. Antibody:Rabbit antiZEB1 recombinant monoclonal antibody [BLR217K] (A700-217). Secondary:HRP-conjugated goat antirabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of mouse ZEB1 by immunohistochemistry Sample: FFPE section of mouse Renca tumor. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR217K] (A700-217). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [BLR217K] - BSA free (ab314092)
Detection of human ZEB1 by immunohistochemistry. Sample: FFPE section of glioblastoma. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR217K] (A700-217). Secondary: HRP-conjugated goat antirabbit IgG.